两段发酵法培养重组酿酒酵母生产乙肝表面抗原  

Two-Stage Cultivation of Recombinant Saccharomyces cerevisiae to Produce Hepatitis B Surface Antigen

作　　者：张凌[1] 周祥山[1] 邵明非[1] 范卫民[1] 张元兴[1] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

出　　处：《华东理工大学学报（自然科学版）》2005年第1期68-71,75,共5页Journal of East China University of Science and Technology

摘　　要：通过具有选择性压力合成培养基的使用和大豆蛋白胨的补加,建立了两段法重组酿酒酵母表达乙肝表面抗原的发酵生产工艺,使总的抗原表达量比原始批培养提高了25%,单位菌体抗原表达量提高了68%。对合成培养基和过程进行了优化,降低了生产成本,使两段发酵法更适合于实际生产。通过测定发酵过程中质粒的稳定性,证明抗原产量的提高是由于质粒稳定性提高所致。Based on the use of defined medium with selective pressure and the feeding of soy peptone, a two-stage fermentation strategy was developed to produce HBsAg by the recombinant Saccharomyces cereviciae. As a result, both the final production of HBsAg and HBsAg yield per cell increased by 25% and 68% respectively. Through the optimization of the defined medium, the two-stage fermentation became economical and potential in practical application. By the measurement of plasmid-bearing cells, it was shown experimentally that the increase of antibody expression was due to the improvement of plasmid stability.

关 键 词：重组酿酒酵母 乙肝表面抗原 HBSAG 质粒 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]