RGD-M6P对原代肝星状细胞的影响  

Effect of RGD-M6P on Primary Hepatic Stellate Cells

作　　者：王连升[1] 陈颖伟[1] 张建华[2] 李定国[1] 陆汉明[1] 

机构地区：[1]上海第二医科大学新华医院消化内科,上海200092 [2]上海第二医科大学基础医学院化学教研室

出　　处：《上海第二医科大学学报》2005年第2期112-115,共4页Acta Universitatis Medicinalis Secondae Shanghai

基　　金：国家自然科学基金 (30 1 70 4 1 2 )资助项目

摘　　要：目的探讨精氨酸 -甘氨酸 -天冬氨酸 (RGD)和甘露糖 - 6 -磷酸 (M6P)的复合体 (RGD M6P)对原代肝星状细胞 (HSC)活化及细胞外基质分泌水平的影响。方法应用胶原酶原位灌注法分离原代HSC ,接种培养 4 8h后 ,随机分成空白对照组、转化生长因子β1(TGFβ1)组、M6P组、RGD组和RGD M6P组。培养 5d后 ,应用免疫组化方法检测各组HSCα SMA表达水平变化 ,双抗体夹心ABC酶免测定法检测培养 7d细胞的上清液中Ⅲ型前胶原 (PCⅢ )含量。结果RGD M6P组原代HSC细胞上清液PCⅢ含量和α SMA表达明显低于TGFβ1组和M6P组 (P <0 .0 5 ) ,而与RGD组比较无显著差异。结论RGD M6P显著减少原代HSCα SMA的表达水平 ,明显降低培养上清中PCⅢ含量 。Objective To study the effect of RGD-M6P on activation and ECM secretion of the primary HSC in vitro. Methods HSCs were isolated from rat by in situ collagenase perfusion of liver, and cultured on uncoated plastic plates for 48 h. Then the HSCs were randomly divided into control group, transforming growth factor β 1 group, M6P group, RGD group and RGD-M6P group. Five days later, smooth muscle α-actin(α-SMA) was detected by immunocytochemistry, and type Ⅲ procollagen (PCⅢ) in supernatant was determined by enzyme linked immunoassay. Results The expressions of α-SMA and PCⅢ level were lower in RGD-M6P group than those in M6P group and TGFβ1 group; but there was no significant difference between RGD-M6P group and RGD group. Conclusion The new compound, RGD-M6P, can dramatically inhibit the expression ofα-SMA of the primary HSC and reduce PCⅢ expression, which may open a new approach for the prevention and targeting therapy of liver fibrosis.

关 键 词：RGD HSC PCⅢ Α-SMA 肝星状细胞 TGFΒ1 含量 分泌水平 接种培养 测定法 

分 类 号：R575.2[医药卫生—消化系统]