白黎芦醇对垂体腺瘤GH3细胞的抑制作用  

Inhibitory effect of resveratrol in pituitary GH3 cells.

作　　者：初明[1] 胡志强[1] 魏兰兰[2] 张树卓[3] 胡恩喜[1] 董齐[1] 

机构地区：[1]哈尔滨医科大学附属一院神经外科,150001 [2]哈尔滨医科大学基础医学院 [3]军事医学科学院毒物药物研究所

出　　处：《中华神经外科杂志》2005年第2期105-109,共5页Chinese Journal of Neurosurgery

基　　金：国家自然科学基金资助项目 (编号: 30370516 );黑龙江省卫生厅基金资助项目(2004 090; 2004 179)

摘　　要：　目的　探讨选择性雌激素受体调节剂白黎芦醇对垂体腺瘤GH3细胞中电压依赖性K+电流(IK(V) )和细胞增殖的影响。方法　不同浓度白黎芦醇作用GH3细胞,应用MTT比色法测定细胞增殖,流式细胞仪检测细胞周期分布,运用全细胞膜片钳技术记录和分析白黎芦醇对GH3细胞中IK(V)的作用。结果　白黎芦醇作用GH3细胞 3d后,以浓度效应关系抑制GH3细胞增殖,并使细胞增殖周期中G0 /G1 期阻滞,S期和G2 /M期百分率降低。全细胞膜片钳结果显示,白黎芦醇以浓度和时间效应抑制IK(V)。结论　白黎芦醇抑制GH3细胞增殖可能是通过阻断电压依赖性K+通道起作用。提示可应用选择性抗雌激素药物治疗垂体腺瘤。Objective Resveratrol(RE), a natural phytoestrogen found in grapes and wine, has been reported to have a variety of health benefit properties. However, little is known of its mechanisms of action on ionic currents in pituitary tumor. We investigated its effect of voltage-dependent K+ current(I K(V))and cell proliferation on GH3 cells derived from the radiation-induced rat pituitary tumor. Methods GH3 cells were treated with RE(10μmol/L, 50μmol/L and 100μmol/L RE). The effect of RE on cell proliferation, distribution of cell cycle and I K(V) in GH3 cell were investigated with the aid of MTT assay, flow cytometry and the whole-cell patch-clamp technique respectively. Results After RE treatment for three days, RE decreased cell proliferation in a concentration-dependent manner with an IC 50 value of 50μmol/L.G 0/G 1 phase in cell proliferating cycle was blocked and cell proportion in the S and G 2/M phase decreased significantly. In whole-cell recording experiments, RE caused an inhibition of I K(V) in a concentration-and time-dependent manner. Conclusions The antiproliferative activity of RE may be partially explained by blockade of voltage-dependent K+ channel present in GH3 cells. These results indicate a role for selective antiestrogen agents in treating pituitary adenomas.

关 键 词：白黎芦醇 GH 垂体腺瘤 细胞增殖周期 抑制作用 全细胞膜片钳 G1期阻滞 期中 结论 百分率 

分 类 号：R736.4[医药卫生—肿瘤]