脑胶质瘤中环氧化酶-2和血管内皮生长因子的表达及作用  

Expression of cyclooxygenase-2 and VEGF in human glioma and its role

作　　者：沈剑峰[1] 曹浩强[2] 周永庆[1] 詹仁雅[1] 黄琼[3] 徐庆生[1] 马廉亭[4] 

机构地区：[1]浙江大学医学院附属第一医院神经外科,杭州310003 [2]浙江省嘉兴市第二医院外科 [3]浙江大学医学院病理和法医学研究所 [4]广州军区武汉总医院神经外科

出　　处：《中华实验外科杂志》2005年第3期338-340,i011,共4页Chinese Journal of Experimental Surgery

基　　金：嘉兴市科委基金资助项目(20031038)

摘　　要：目的研究62例脑胶质瘤细胞中环氧化酶(COX)2、血管内皮生长因子(VEGF)和第八因子相关抗原(ⅧRAg)的表达,探讨COX2在脑胶质瘤中的作用。方法运用免疫组织化学方法研究COX2、VEGF和Ⅷ因子相关抗原在脑胶质瘤中的表达。结果COX2在脑胶质瘤细胞中的胞浆染色阳性,和胶质瘤的病理级别正相关(r=0.874,P<0.01);在坏死区域周围有COX2高表达的肿瘤细胞积聚;肿瘤组织中的血管周围有COX2染色阳性的肿瘤细胞聚集。脑胶质瘤中COX2和VEGF的表达(r=0.4578),COX2的表达和MVD之间(r=0.3918)呈正相关性,差异有统计学意义(P<0.01)。结论COX2的表达和脑胶质瘤的病理分级成正相关,和脑胶质瘤的发生和发展有关系;COX2的表达和VEGF成正相关,COX2和微血管密度成正相关,COX2的表达可促进肿瘤组织新生血管的生成;COX2可作为判断脑胶质瘤恶性度的辅助生物学标志。Objective To evaluate the expression of Cyclooxygenase-2 (COX-2),vascular endothelial growth factor (VEGF) and factor Ⅷ in 62 surgically removed human gliomas and the role of COX-2 in human glioma.Methods By using immunohistochemistry,the expression of COX-2,VEGF and factor Ⅷ in human glioma was detected.Results In the low grade astrocytoma,COX-2 was low and the tumor cells were mainly light-stained.COX-2 was strongly expressed in glioblastoma.COX-2 expression in anaplastic astrocytoma was moderate in contrast to the low grade astrocytoma and glioblastoma.COX-2 was positively correlated with glioma (r=0.874,P<0.01).The immunoreactive score of COX-2 and VEGF,also the MVD were higher in glioblastoma than in anaplastic and low-grade astrocytoma.COX-2 was positively correlated with VEGF (r=0.457?8,P<0.01) and MVD (r=0.391?8,P<~0.01). Conclusion Immunohistochemical determination of COX-2 expression cell types helps to understand possible roles of COX-2 in tumor tissues.Also COX-2 enhances the expression of VEGF and Factor Ⅷ.This evidence suggests that COX-2 expression in the glioma could be associated with astrocytoma progression and also stimulation of angiogenesis.COX-2 is the promotor of the human glioma.

关 键 词：COX-2 脑胶质瘤细胞 高表达 血管内皮生长因子 VEGF 环氧化酶-2 阳性 聚集 积聚 坏死 

分 类 号：R739.41[医药卫生—肿瘤] R735[医药卫生—临床医学]