海枣曲霉β-木糖苷酶的提纯和性质  

PURIFICATION AND PROPERTIES OF β-XYLOSIDASE FROM ASPERGILLUS PHOENICIS

作　　者：曾宇成 张树政[1] 

机构地区：[1]中国科学院微生物研究所

出　　处：《微生物学报》1993年第6期427-433,共7页Acta Microbiologica Sinica

摘　　要：从海枣曲霉麦麸培养物抽提液中，通过聚乙二醇６０００－磷酸钾缓冲液双水相分离，相继用Ｓｅｐｈａｄｅｘ Ｇ－１００凝胶过滤、ＤＥＡＥ－Ｓｅｐｈａｄｅｘ Ａ－５０离子交换柱层析、羟基磷石吸附层析、ＤＥＡＥ－Ｓｅｐｈａｄｅｘ Ａ－５０离子交换层析、ＳＥ－Ｓｅｐｈａｄｅｘ Ｃ－５０离子交换层析以及最适温度为６５℃，在ｐＨ３．５－６．５之间稳定，酶保温３０分钟时的半失活温度（ｔ１／２）为６８℃。A β-Xylosidase has been purified to PAGE homogeneity from the wheat branculture of Aspergillus phoenicis.by PEG 6000-phosphate buffer aqueous twophaseseparation and successive chromatography on Sephadex G-100,DEAE-Sephadex A-50,hydroxyapatite,DEAE-Sephadex A-50,SE-Sephadex C-50 and Sephadex G-50columns.The enzyme showed optimum activity at pH 3.5 and 65(?),it was stable in thepH range 3.5—6.5.The molecular weight of the enzyme was 95000 as determined bySDS-PAGE and concentration gradient-PAGE.The isoelectric point was estimated to bepI 4.4.The enzyme showed the highest activity toward pNP-β-Xyloside.The K_m andthe V_(max) values were 0.63mmol/L,and 410μmol·min^(-1)·mg^(-1),respectively.Theenzyme was strongly inhibited by Hg^(2+),Ag^+ and D-Xylose.The K_1 for D-Xylose wasabout 7.5 mmol/L,as determined by Lineweaver-Burk plot and Dixon plot.

关 键 词：海枣曲霉 Β-木糖苷酶 提纯 

分 类 号：Q939.506[生物学—微生物学]