黄瓜前中期染色体C-分带及其制备方法研究  

C-banding of Prometaphase Chromosomes in Cucumber and the Procedure for Slide Preparation

作　　者：钱春桃[1] 陈劲枫[1] 罗向东[1] 曹清河[1] 雷春[1] 

机构地区：[1]作物遗传与种质创新国家重点实验室,南京农业大学园艺学院南京210095

出　　处：《园艺学报》2004年第6期814-816,共3页Acta Horticulturae Sinica

基　　金：国家高技术研究发展计划专项(2002AA241251;2002AA207012);国家自然科学基金资助项目(30170644;30470120);教育部跨世纪优秀人才培养计划项目;南京农业大学青年科技创新基金项目(KJ04009)

摘　　要：以活体种子根为材料,采用放线菌酮预处理,直接获得了清晰的黄瓜前中期染色体C-分带。黄瓜前中期染色体的长度介于3～8μm,单套染色体组具有21条稳定的C-带,包括12条末端带、7条着丝点带、1条中间带和1条随体带。还探讨了影响前中期C-分带制备的关键因素,提出了黄瓜前中期染色体C-分带的实用制备方法。Clear C-bands at prometaphase were obtained in cucumber(Cucumis sativus L.,2n=2x=14)when cycloheximide was applied to the germinating roots in vivo as pretreatment. Chromosome length at prometaphase varied from 3 to 8μm. Haploid complement had 21 distinct and stable C-bands, including 12 telomeric-, 7 centromeric-,1 intercary- and 1 satellite-bands. Some factors affecting the band formation were investigated and a practical procedure of C-banding of prometaphase chromosome was proposed.

关 键 词：黄瓜 染色体 C-分带 前中期 

分 类 号：S642.2[农业科学—蔬菜学]