胰岛素样生长因子-1对大鼠肝纤维化形成的影响  

Effect of insulin-like growth factor-l on hepatic fibrosis in rats

作　　者：万荣[1] 吴云林[1] 乔敏敏[1] 章永平[1] 付爱芬[2] 辛美珍 孔雷[3] 张奕[3] 刘炳亚[3] 

机构地区：[1]上海第二医科大学附属瑞金医院消化科,上海市200025 [2]上海第二医科大学病理解剖教研室,上海市200025 [3]上海第二医科大学附属瑞金医院上海外科研究所,上海市200025

出　　处：《世界华人消化杂志》2005年第2期211-213,共3页World Chinese Journal of Digestology

基　　金：日本Asahi医学发展项目基金资助项目;No.~~

摘　　要：目的:探讨胰岛素样生长因子-1对大鼠肝纤维化形成的影响.方法:50只SD大鼠随机编入4组,正常组和橄榄油组各10只,模型组15只,IGF-1干预组15只.40%CCL4橄榄油溶液制备大鼠肝纤维化模型,4wk后,以IGF-12ug/kg干预,共2wk.观察大鼠存活率、体重变化及纤维化形成率;光镜下观察大鼠肝组织病理及胶原纤维变化;采用Western-blot法检测肝组织中α-肌动蛋白(α-SMA)含量.结果:IGF-1干预组大鼠存活率高于模型组,体重(301.5±32.4g)明显高于模型组(252.1±24.1g,P<0.05),肝纤维化形成率较模型组低,光镜下肝组织纤维化程度明显改善,胶原含量明显下降.IGF-1干预组中肝组织α-SMA含量明显低于模型组.结论:IGF-1具有抗大鼠肝纤维化作用,这可能与其改善肝功能、抑制HSC活化等有关.AIM: To investigate the effect of insulin-like growth factor-Ⅰ(IGF-1) on hepatic fibrosis in rats and its possible mechanism. METHODS: Fifty Sprague-Dawley rats were randomly divided into 4 groups: normal group (n=10), olive oil group (n = 10), model group (n = 15) and IGF-1 group (n = 15). The hepatic fibrosis model was induced by 40% CCI4. The rats with hepatic fibrosis were injected with IGF-1 subcutane-ously four weeks later (2 μg/kg, every other day for 2 weeks). The survival rate, fibrotic degree and body weight of the rats were inspected. The histopathological changes of col-lagenous fiber were examined under optical microscope. The content of α-smooth muscle actin (α-SMA) in the liver tissue were detected by Western-blotting. RESULTS: In comparison with those in model group, the weights of the rats in IGF-1 group were significantly higher (252.1±24.1 g vs301.5±32.4 g, P<0.05); the survival rate was higher and the fibrotic degrees were remarkably lower; the content of collagen and α-SMA in the liver tissue were significantly reduced. CONCLUSION: IGF-1 can protect rats against hepatic fibrosis, which may be related to its role in improving hepatic function and inhibiting HSC activation.

关 键 词：胰岛素样生长因子-1 大鼠 肝纤维化 Α-肌动蛋白 胶原纤维 

分 类 号：R575.2[医药卫生—消化系统]