超临界流体萃取佛手挥发油的气相色谱/质谱分析  

Supercritical carbon dioxide extraction of bergamot essential oil and its components

作　　者：金晓玲[1] 何新霞[1] 杜红岩[2] 俞鲁波[1] 

机构地区：[1]浙江师范大学化学与生命科学学院,浙江金华321004 [2]中国林业科学院经济林研究中心,河南郑州450003

出　　处：《浙江师范大学学报（自然科学版）》2005年第1期61-65,共5页Journal of Zhejiang Normal University:Natural Sciences

摘　　要：采用超临界流体CO2 萃取法提取新鲜佛手和干佛手的挥发油,经气相色谱 质谱联机分析,新鲜佛手挥发油的得率为 16. 2 mL/kg,鉴定出 34 个组分,主成分为丁二醇,占 16. 28%;干佛手挥发油的得率为9.6 mL/kg,分2个不同时段用不同的条件萃取,第1个时段鉴定出51个组分,第 2 个时段鉴定出40个组分,干佛手挥发油的主成分为5,7 二甲氧基香豆素,占19.49%.而且,干佛手第2个时段的挥发油中含有较多的中长链脂肪酸,总量达26.47%.In order to analyze the chemical composition and their relative contents in essential oil of bergamot, bergamot essential oil was extracted by supercritical carbon dioxide(SC-CO-2). The chemical compositions and their relative contents in essential oil of bergamot were analyzed by GC-MS. The subject were fresh bergamot and dried bergamot from Jinhua. They were extracted respectively. The results were: 34 compounds were identified from fresh bergamot oil and the yield was (16.2 mL5kg^(-1)). Dried bergamot was extracted by SC-CO-2 at two different period with different conditions, the yield was 9.6 mL5kg^(-1). 51 compounds were identified of the first period and 40 compounds were identified of the second period. They were showed that the technology of supercritical carbon dioxide(SC-CO-2) extraction can significantly increase the yield and the quality of bergamot essential oil. The main components of fresh bergamot oil was butanediol (16.28% ) and dried bergamot oil was coumarin, 5,7-dimethoxy-((19.49%)). The rate of inside long chain fatty acids of dried bergamot oil was 26.47% at second period.

关 键 词：佛手挥发油 气相色谱/质谱分析 时段 经气 取法 鉴定 超临界流体萃取 组分 丁二醇 得率 

分 类 号：R974[医药卫生—药品] TQ657[医药卫生—药学]