宫内生长迟缓大鼠脂肪组织肿瘤坏死因子α mRNA表达与胰岛素抵抗关系的研究  

Expression of tumor necrosis factor α mRNA in adipose cell of intrauterine growth retarded rats and its relation to insulin resistance

作　　者：黄婷婷[1] 丘小汕[1] 杜敏联[1] 沈振宇[1] 柯志勇[1] 赖峰[1] 

机构地区：[1]广州中山大学第一附属医院儿科,510080

出　　处：《中华儿科杂志》2005年第1期39-43,共5页Chinese Journal of Pediatrics

基　　金：广东省重点科技基金资助项目 (99M0 4815G)

摘　　要：目的　探讨内脏脂肪组织肿瘤坏死因子α(TNF α)的mRNA表达与宫内生长迟缓(IUGR)大鼠成年后胰岛素抵抗 (IR)发生的关系 ,以及生后早期不同的营养模式的远期影响。方法　用母鼠全程饥饿法建立IUGR动物模型。 32只IUGR新生雌鼠随机分为 4组 :IUGR模型组 (S/N组 )给予常规饮食 ,IUGR高热能饮食组 (A组 ) ,IUGR高蛋白高热能饮食组 (B组 ) ,IUGR高蛋白质等热能饮食组 (C组 )。各组幼鼠在 3周哺乳期间母鼠分别摄取上述饲料。第 4周起各组幼鼠断乳后全部改为自由进食常规饮食至实验结束。另以正常母鼠所生正常新生雌鼠 8只为正常对照组 (C/N组 )生后给予常规饮食喂养至实验结束。各组大鼠在 4 8周 (中老年期 )时分别测定体重、肾周脂肪重量、脂肪组织TNF αmRNA表达 ,计算ISI(胰岛素敏感指数 )并分析其与TNF αmRNA表达量及肾周脂重、脂重 /体重的相关性。结果　IUGR模型组及IUGRA、B组胰岛素敏感性下降 ,并且脂肪组织TNF αmRNA表达及肾周脂重、脂重 /体重显著高于正常对照组 (P <0 0 5或 0 0 1) ;IUGR的C组胰岛素敏感性及TNF αmRNA表达量、脂重 /体重与正常对照组差异无统计学意义 (P >0 0 5 )。脂肪组织TNF αmRNA水平与肾周脂肪重量、脂重 /体重量呈正相关 ,与ISI呈负相关。脂重 /体重与ISI呈负相关。?Objective To investigate the association between the expression of turnor necrosis factor α (TNF α) mRNA in fat tissue of intrauterine growth retarded (IUGR) rats and insulin resistance, and the long term effects of early different nutritional diet Methods The IUGR rat model was established by food restriction of pregnant rats A total of 32 newborn IUGR rats were randomly divided into 4 groups: IUGR model (S/N) group,IUGR high caloric diet (A) group, IUGR high caloric and high protein diet (B) group, IUGR high protein diet (C) group Only the mother rats were given those different diets individually, and all IUGR newborn pups were lactated for 3 weeks From the beginning of the 4 th week, all IUGR pups were weaned and fed with normal diet till the end of the experiment Eight normal birth weight newborn rats were used as the control group fed with the normal diet Weight, perirenal fat weight, fasting glucose and insulin concentration and quantified TNF α mRNA expression in adipose cell were measured at the 48 th week The insulin sensitive index (ISI) and the relation index between TNF α mRNA and fat weight,fat weight/body weight (fw/bw) ratio and ISI were calculated Results ISI of IUGR model group, IUGR A and B groups was lower than normal control group, while perirenal fat weight,fw/bw and the expression of TNF α mRNA in adipose cells were all significantly higher ( P <0 05 or 0 01) There were no significant differences in these indexes between IUGR C group and normal control groups( P >0 05) A positive correlation was found between TNF α mRNA and fat weight and fw/bw ( r 1 =0 755, r 2 =0 782, P =0 000) Significant inverse associations between ISI and TNF α mRNA( r =-0 556, P =0 000) and fw/bw( r =-0 513, P =0 02) were also found Conclusion The occurrence of insulin resistance in IUGR rats is possibly associated with central obesity and accumulation of the abdominal fat and adipose cell over expression of TNF α The adi

关 键 词：IUGR 胰岛素抵抗 TNF—α MRNA表达 饮食 脂肪组织 大鼠 营养模式 体重 母鼠 

分 类 号：R363[医药卫生—病理学]