异丙酚对家兔肝缺血/再灌注后抗氧化能力改变的影响  

EFFECT OF PROPOFOL ON ANTI-OXIDATION CAPABILITY IN THE ISCHEMIA/REPERFUSION INJURY AFTER HEPATIC ISCHEMIA IN RABBITS

作　　者：王万铁[1] 林丽娜[2] 王卫[1] 李东[3] 

机构地区：[1]温州医学院病理生理学教研室 [2]温州医学院附属第一医院麻醉科 [3]温州医学院检验系分析中心,浙江温州325027

出　　处：《中国应用生理学杂志》2005年第1期26-29,共4页Chinese Journal of Applied Physiology

基　　金：浙江省跨世纪学术和技术带头人培养基金资助(992 0 86) ;温州市"5 5 1人才工程"培养基金资助 (98113 ) ;浙江省卫生厅科研基金资助 (98A0 87)

摘　　要：目的 :探讨氧自由基 (OFR)在肝缺血 /再灌注损伤 (HI/RI)中的作用及异丙酚对其的影响。方法 :实验兔随机分为假手术对照组、肝缺血 /再灌注组和肝缺血 /再灌注加异丙酚治疗组 ,分别在肝缺血前、缺血 4 5min、再灌注4 5min共 3个时相点 ,检测血浆及肝组织超氧化物歧化酶 (SOD)活性、黄嘌呤氧化酶 (XO)活性、丙二醛 (MDA)浓度及谷丙转氨酶 (ALT)值 ,并行肝组织电镜观察。结果 :肝缺血 /再灌注期间 ,血浆XO、MDA及ALT显著高于、SOD明显低于假手术对照组 (P <0 .0 5和P <0 .0 1) ;肝组织XO及MDA显著高于、SOD明显低于假手术对照组(P <0 .0 5和P <0 .0 1) ;肝组织超微结构发生异常改变。异丙酚可逆转上述指标的异常变化 ,与肝缺血 /再灌注组相比有显著性差异 (P <0 .0 5和P <0 .0 1)。结论 :OFR在HI/RI发生发展中起介导作用 ;异丙酚可通过降低氧自由基水平 (增强SOD活性、减弱XO活性 ) ,拮抗脂质过氧化反应 (降低MDA浓度 ) ,从而减轻HIRI。Aim: To investigate the role of oxygen free radicals (OFR) in hepatic ischemia and reperfusion injury (HI/RI) and effect of propofol on them. Methods: The rabbits were randomly divided into three groups(n=10),sham operated group(Control),HIR group(I/R) and HIR+ propofol group(PRO).Changes of several parameters which included malondialdehyde (MDA), superoxide dismutase (SOD), xanthine oxidase (XO) and alanine aminotransferase(ALT) were measured before ischemia,45 minutes after ischemia and 45 minutes after reperfusion in plasma.Meanwhile MDA concentration,SOD,XO activities and ALT value in liver tissue were measured,and the ultrastructure changes in liver tissue were observed under electron microscope at 45 minutes after reperfusion. Results: As compared with group control, XO,MDA and ALT increased and SOD decreased during HI/RI(P<0.05 and P<0.01) in plasma, and XO,MDA increased as well as SOD decreased at 45 minutes after reperfusion (P<0.05 and P<0.01) in liver tissue and there were abnormal changes of the hepatic ultrastructure at 45 minutes after reperfusion.Propofol reversed the results of mentioned indices as above markedly(P<0.05 and P<0.01).Conclusion: OFR is an important factor during HI/RI, propofol may attenuate hepatic ischemia-reperfusion injury by dropping OFR level( raising SOD activity and dropping XO activity) and antagonizing lipid pe- roxidation (reducing MDA content).

关 键 词：缺血/再灌注损伤 肝脏 氧自由基 异丙酚 

分 类 号：R363.27[医药卫生—病理学] R965[医药卫生—基础医学]