河豚毒素停搏液减轻大鼠心肌细胞内钙超载作用的实验研究  

Tetrodotoxin cardioplegia solution alleviates intracellular calcium overload in rat cardiomyocytes

作　　者：杨朝坤[1] 杨双强[1] 谭松涛[1] 汪斌[1] 陈力[1] 

机构地区：[1]重庆医科大学附属第一医院胸心外科,重庆400016

出　　处：《第三军医大学学报》2005年第3期192-195,共4页Journal of Third Military Medical University

摘　　要：目的　观察Na+ 通道阻滞剂河豚毒素 (TTX)停搏液对离体大鼠心肌细胞内游离Ca2 + 浓度的影响。方法　成年Wistar大鼠心脏 ,用酶解法分离成具有搏动性的心室肌细胞悬液 ,随机分成基础组、STH2组和TTX组 ,STH2组和TTX组分别应用STH2停搏液和TTX停搏液处理 ,建立模拟缺血 /再灌注损伤的停搏 /复搏细胞模型 ,激光扫描共聚焦显微镜测定各组细胞不同时期的 [Ca2 + ] i。用倒置显微镜观察细胞搏动情况和形态学变化。结果　TTX组和STH2组细胞复搏后[Ca2 + ] i 均明显高于基础组 (P <0 .0 1) ,但TTX组 [Ca2 + ] i 明显低于STH2组 (P <0 0 1) ;在停搏期间 ,TTX组细胞 [Ca2 + ] i 上升速度和幅度明显低于STH2组 ;STH2组和TTX组细胞复搏后搏动频率无明显差别 (P >0 0 5 ) ;形态学观察 ,TTX组复搏后具有正常活力的杆形心肌细胞比例高于STH2组 (P <0 0 1)。结论　河豚毒素停搏液能减轻心肌细胞内Ca2 + 超载和防止缺血再灌注损伤。Objective To investigate the effects of sodium channel inhibitor, tetrodotoxin (TTX) cardioplegia solution, on intracellular free Ca 2+ concentration in isolated cardiomyocytes of rats. Methods Ventricular beating myocytes isolated from adult rat hearts by enzymatic dissociation were randomly divided into Base, STH2, and TTX groups. Myocytes in STH2 and TTX groups were treated with St. Thomas Ⅱ and TTX cardioplegia solution respectively for the establishment of the arrest/re-beating cell models of mimic ischemia-reperfusion injury (MIRI). Tetrodotoxin cardioplegia solution alleviates intracellular calcium overload in rat cardiomyocytes$$$$ YANG Chao-kun, YANG Shuang-qiang, TAN Song-tao, WANG Bin, CHEN Li (Department of Cardiothoracic Surgery, First Affiliated Hospital, Chongqing University of Medical Sciences, Chongqing 400016, China) Abstract: Objective To investigate the effects of sodium channel inhibitor, tetrodotoxin (TTX) cardioplegia solution, on intracellular free Ca 2+ concentration in isolated cardiomyocytes of rats. Methods Ventricular beating myocytes isolated from adult rat hearts by enzymatic dissociation were randomly divided into Base, STH2, and TTX groups. Myocytes in STH2 and TTX groups were treated with St. Thomas Ⅱ and TTX cardioplegia solution respectively for the establishment of the arrest/re-beating cell models of mimic ischemia-reperfusion injury (MIRI). [Ca 2+ i of cardiomyocytes at different stages in each group was measured by laser scanning confocal microscopy (LSCM). The beating and morphology of cardiomyocytes were observed by the inverted microscope. Results [Ca 2+i after re-beating in both STH2 and TTX groups was higher than that in Base group (P<0.01), but [Ca 2+ i in TTX group was lower than that in STH2 group (P<0.01). During arrest, the elevation of [Ca 2+ i in TTX group was lower than that in STH2 group. There was no difference in beating rate after re-beating between STH2 and TTX groups (P>0.05). Morphologically, the ratio of normal a

关 键 词：心脏停搏液 极化停搏 河豚毒素 钠通道 心肌细胞 Ca^2+ 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学] R654.1[医药卫生—基础医学]