PD98059对11,12-EET及缺血预处置大鼠心功能保护作用的影响  

PD98059 on Heart Function Endured11,12-EET and Ischemia Preconditioning

作　　者：王红霞[1] 曾翔俊[1] 闫丽[1] 芦玲巧[1] 张立克[1] 

机构地区：[1]首都医科大学病理生理学教研室

出　　处：《首都医科大学学报》2005年第1期48-50,共3页Journal of Capital Medical University

基　　金：北京市教委科技发展基金 (KM 2 0 0 5 10 0 2 5 0 0 9)

摘　　要：为观察PD980 5 9对给予 11,12 EET或缺血预处置在体大鼠再灌注心功能的影响 ,了解磷酸化ERK1/ERK2的表达在上述心脏保护中的作用 ,使用雄性Wistar大鼠 ,通过结扎 (6 0min)和松开 (30min)冠状动脉左前降支 ,复制缺血 /再灌注模型 ;采用缺血 5min ,再灌注 5min 2次造成缺血预处置 ;6 .2 4× 10 -8mol/L 11,12 EET通过静脉给予。计算机记录再灌注过程中心功能的变化 ;实验结束时取心肌采用Western Blot法测定ERK1/ERK2的表达。结果显示 :再灌注 30min ,I/R组的 +dp/dtmax(收缩期左心室内压上升的最大变化速率 )、-dp/dtmax(舒张期左心室内压下降的最大变化速率 )和LVDP(左心室发展压 )均显著低于SI +I/R组和EET +I/R组 (P <0 .0 5 ) ;SI +I/R +PD组的 +dp/dtmax、-dp/dtmax及LVDP均明显低于SI+I/R组 (P <0 .0 5 ) ,EET +I/R +PD组的 +dp/dtmax、-dp/dtmax及LVDP明显低于EET +I/R组(P <0 .0 5 )。研究表明 :6 .2 4× 10 -8mol/L 11,12 EET及缺血预处置均有保护心功能的作用 ;这种保护作用通过激活缺血 /再灌注时ERK1/ERK2的表达而实现。The aim was to investigate the effect of PD98059 on heart function endured 11, 12-EET a nd ischemia preconditioning and know about the effect of the expression of phosp horylated ERK1/ERK2 on the cardiac protection. Adopting ligating 60 min a nd releasing 30 min to make a ischemic/reperfusion model; ligating 5 min and rel ea sing 5 min (two times) to replicate ischemic preconditioning model. Observing th e changing of heart function during reperfusion process and detecting the expressi on of ERK1/ERK2 through western blot method. There was significant di fferen ce contrasting the heart function I/R group with SI+I/R and EET+I/R group, and t he heart function was significantly decreased after PD98059 infusion. The phosph orylated expression of ERK of I/R group was significantly lower than SI+I/R and E ET+I/R group, and the phosphorylated expression of ERK was significantly decreas ed after PD98059 infusion. It suggests that 6.24×10 -8 mol/L 11,12-EET h as the effect of protecting myocardium function that is inducted by increasing the expression of phosphorylated ERK1/ERK2.

关 键 词：缺血 心功能 再灌注 PD98059 ERK 保护作用 大鼠 左心室内压 雄性 WESTERN-BLOT 

分 类 号：R743[医药卫生—神经病学与精神病学] R541[医药卫生—临床医学]