大鼠脑出血模型血肿周围继发损害的分子生物学机制研究  

Molecular Biology Mechanism for Secondary Injury to Perihematoma in ICH Rats

作　　者：赵性泉[1] 王拥军[1] 王春雪[1] 周剑[2] 王元身[3] 姬蔓[3] 

机构地区：[1]首都医科大学附属北京天坛医院神经内科 [2]武警总医院影像室 [3]首都医科大学神经科学研究所

出　　处：《首都医科大学学报》2005年第1期77-80,共4页Journal of Capital Medical University

摘　　要：通过大鼠脑出血模型血肿周围组织HSP70、NF κB、Bax和Bcl 2免疫组化研究 ,探讨脑出血血肿周围组织继发性损害的可能机制。制备大鼠脑出血模型 ,实验组分成 1h、3h、12h、2 4h、4 8h、72h及 7d 7个小组 ;生理盐水对照组分成 3h、2 4h及 72h 3个小组 ,在血肿周边区及同侧皮质区分别取脑组织行HSP70、NF κB、Bax和Bcl 2抗体免疫组织化学染色 ,采用HP10 0 0高清晰度彩色病理图像分析系统计数免疫反应阳性细胞数 ,并与生理盐水对照组进行比较。发现 :实验组的HSP70和NF κB在血肿侧术后 72h表达达高峰 (P <0 .0 1) ;血肿侧各时间点HSP70和NF κB阳性细胞数与生理盐水对照组相比差异有统计学意义 (P <0 .0 1) ;实验组Bcl 2在术后 12h表达达高峰 (P <0 .0 1) ,而Bax表达在 2 4h达高峰 (P <0 .0 1) ,二者的血肿侧阳性细胞数与生理盐水对照组相比差异有统计学意义 (P <0 .0 1)。结果提示 :脑出血后血肿周围组织HSP70、NF κB、Bcl 2和Bax蛋白发生动态变化 ,参与了血肿周围继发性损害的病理过程。The aim was to study possible molecular biology mechanism of secondary lesion to perihematom a tissue in intracerebral haemorrhage (ICH) rats by testing HSP70, NF-κB , Bax, Bcl-2 of surrounding tissue of hematoma through immunohistochemistry met hod. Sp rague-Dawley male rats were subjected to ICH models. They were randomly divide d into test groups and control groups. Test groups were divided into 7 subgroups at 1 h, 3 h, 12 h, 24 h, 48 h, and 72 h after ICH, control groups were divided into 3 subgroups at 3 h, 24 h, 72 h after normal saline injection. HSP70, NF-κ B, Bcl-2, Bax were tested by immunohistochemistry staining at perihematoma area . Positive cells were picked up by HP1000 high-definition colorful pathology im aging analysis system and compared with the control subgroups. HSP70 and NF-κB expressed the most at the hematoma side at 72 h after surgery (P<0.01); The y demonstrated significant difference among subgroups compared with control subgro ups. Bcl-2 expressed the highest at 12 h after surgery (P<0.01) and Bax at 24 h after injection (P<0.01); Bcl-2 and Bax showed significant difference among subgroups compared with control groups (P<0.01). The result suggests t hat dynamic alternation of HSP70, NF-κB, Bcl-2 and Bax participates and plays an important role in the pathologic process of secondary injury to perihematoma tissue.

关 键 词：血肿 脑出血 HSP70 NF-κB 对照组 生理盐水 Bcl-2 时间点 差异 提示 

分 类 号：R743.34[医药卫生—神经病学与精神病学] R651[医药卫生—临床医学]