谷氨酸和白细胞介素1β致痫大鼠海马PKAcβ、PKCα的免疫反应变化  

Changes of PKAcβ and PKCα immunoreaction in hippocampus of epileptic rats induced by L-glutamate and IL-1β

作　　者：李莉[1] 李正莉[1] 朱长庚[1] 刘庆莹[1] 魏瑛[1] 童逸龄[1] 

机构地区：[1]华中科技大学同济医学院基础医学院解剖学教研室脑研究室,武汉430030

出　　处：《解剖学杂志》2005年第1期50-52,共3页Chinese Journal of Anatomy

基　　金：湖北省自然科学基金 (2 0 0 1ABB1 4 2 )

摘　　要：目的 :研究谷氨酸和白细胞介素 1β(IL 1β)致痫大鼠海马PKAcβ、PKCα的免疫反应变化。 方法 :大鼠于脑立体定位仪上行侧脑室注射 ,A组为生理盐水对照组 ;B组注射阈下致痫量的L 谷氨酸钠 ;C组注射致痫剂量的L 谷氨酸钠 ;D组单纯注射IL 1β;E组联合注射IL 1β和阈下致痫量的L 谷氨酸钠。注射完毕 1h后用SABC法显示 5组大鼠海马的PKAcβ、PKCα的免疫反应变化。结果 :C、D、E 3组大鼠均有明显的痫性发作 ,且海马PKAcβ、PKCα的免疫反应均明显强于A组。 结论 :在谷氨酸和IL 1β的致痫活动中 ,PKAcβ、PKCα参与了其细胞内信号转导过程。Objective: To investigate the changes of the immunoreaction of protein kinase A catalytic β (PKAcβ) and protein kinase C α (PKCα) in the hippocampus of epileptic rats induced by L-glutamate and IL-1β. Methods: Forty adult SD male rats were randomly divided into 5 groups (n=8): group A (0.9% saline, i.c.v.), group B (L-glutamate, 750 μg/kg, i.c.v.), group C (L-glutamate, 1500 μg/kg, i.c.v.), group D (IL-1β, 2 500 U/kg, i.c.v.) and group E (IL-1β 2 500 U/kg + L-glutamate 750 μg/kg, i.c.v.). The behavior of the animals was observed and the immunoreaction of PKAcβ and PKCα in the brain was displayed by immunohistochemistry SABC staining method. Results: The rats of group C and E had grade Ⅳ to grade Ⅴ epileptic activities. There were higher density of the immunoreaction of PKAcβ and PKCα in group C, D, E than in group A (P<0.05). Conclusion: PKA and PKC may be involved in the signal transduction of the epileptic activity induced by IL-1β and L-glutamate.

关 键 词：PKC 免疫反应 侧脑室注射 IL-1β 海马 致痫大鼠 白细胞介素-1β PKA 细胞内信号转导 SABC法 

分 类 号：R742.1[医药卫生—神经病学与精神病学] R743[医药卫生—临床医学]