舟山眼镜蛇毒细胞毒素-Ⅻ的抗肿瘤作用  

Antitumor Activity of Cytotoxin-ⅩⅡ from Naja atra venom

作　　者：吴国土[1] 张明芳[1] 许云禄[2] 

机构地区：[1]福建医科大学生理学与病理生理学系,福州350004 [2]福建医科大学药理学系蛇毒研究所,福州350004

出　　处：《福建医科大学学报》2005年第1期35-37,共3页Journal of Fujian Medical University

基　　金：福建省教育厅科研基金资助项目 (K0 40 5 5 )

摘　　要：目的　了解舟山眼镜蛇毒细胞毒素 - (CTX- )的抗肿瘤作用。　方法　应用磺胺邻二甲氧嘧啶(SRB)法观察不同浓度 CTX- 对人肝癌 Bel74 0 4细胞和人早幼粒白血病 HL - 6 0细胞的生长抑制作用。观察CTX- 0 .4 ,0 .2 ,0 .1mg/ kg腹腔注射对 U 14荷瘤小鼠瘤块质量的影响并计算抑瘤率 ;CTX- 0 .4和 0 .2 m g/kg静脉注射对艾氏腹水癌 (EAC)小鼠腹水形成的影响并计算腹水减少率。　结果　 CTX- 可明显抑制人肝癌Bel74 0 4细胞和人早幼粒白血病 HL - 6 0细胞的生长 ,其 IC50 分别为 (1.12± 0 .13)和 (1.6 1± 0 .18)μg/ m L。 CTX- 0 .4 ,0 .2和 0 .1m g/ kg腹腔注射× 10 d,对宫颈癌 U14抑瘤率分别为 30 .5 0 % (P<0 .0 1) ,19.0 2 % (P<0 .0 5 )和12 .0 8% (P>0 .0 5 ) ;CTX- 0 .4和 0 .2 mg/ kg静脉注射× 3d对 EAC小鼠腹水形成减少率分别为 2 5 .5 % (P<0 .0 5 )和 14 .5 % (P>0 .0 5 )。　结论　舟山眼镜蛇毒细胞毒素 - 具有明显的抗肿瘤作用。Objective To investigate the antitumor effects of cytotoxin-ⅩⅡ(CTX-ⅩⅡ) isolated from the venom of Naja atra. Methods The inhibition effects of cytotoxin-ⅩⅡ on the cultured Bel7404 cells and HL-60 human leukemia cells were observed by sulforhodamine B(SRB) method. Uterus cervix cancer U14 bearing mice was treated with CTX-ⅩⅡ(0.4, 0.2 and 0.1 mg/kg, ip). Ehrilich ascites carcinoma cells(EAC) bearing mice was treated with CTX-ⅩⅡ(0.4 and 0.2 mg/kg, iv). Results The cytotoxin-ⅩⅡ inhibited the cultured Bel7404 cells and HL-60 human leukemia cells, IC 50 of CTX-ⅩⅡ on Bel7404 cells and HL-60 human leukemia cells was (1.12±0.13) and (1.61±0.18) μg/mL, respectively. CTX-ⅩⅡ 0.4, 0.2 and 0.1 mg/kg, ip×10 d inhibited the growth of U14 in mice were 30.50%(P<0.01), 19.02%(P<0.05), 12.08%(P>0.05); CTX-ⅩⅡ 0.4, 0.2 mg/kg, iv×3 d also showed regression of ascetic tumors(EAC) in mice 25.5%(P<0.05) and 14.5% respectively(P>0.05). Conclusion cytotoxin-ⅩⅡ exhibited significant antitumor effect in cultured tumor cell or in tumor-bearing mice.

关 键 词：眼镜蛇毒液类 细胞毒素类 肿瘤细胞 培养的 凋亡 

分 类 号：R73-3[医药卫生—肿瘤]