机械张应力对兔腭中缝血管内皮生长因子mRNA表达的影响  

Expression of VEGF mRNA during sutured distraction osteogenesis in rabbits

作　　者：张卫兵[1] 王林[1] 仲伟洁[1] 马俊青[1] 杜娟[1] 严斌[1] 

机构地区：[1]南京医科大学口腔医学院正畸科,南京210029

出　　处：《口腔医学》2005年第1期49-51,共3页Stomatology

基　　金：江苏省 13 5工程项目;江苏省青蓝工程项目 ( 8710 )

摘　　要：目的　观察在快速扩张兔腭中缝的扩张期和固定期血管内皮因子 (VEGF)mRNA表达情况。方法　用螺旋分裂基托扩大矫治器 (Haas矫正器 )扩张兔上牙弓 ,每 12h 0 .2 5mm ,共扩张牵引 14d ,分别于即刻 ,第 1、2、3、4、6周取材 ,标本常规进行逆转录聚合酶链反应 (RT -PCR)检测VEGFmRNA的表达。结果　第 1、2、3、4、6周VEGFmRNA表达量实验组均高于对照组。在实验组VEGFmRNA各组间的变化趋势为随着机械应力快速扩张 ,VEGFmRNA的表达量逐渐上升 ,固定 1周达峰值 ,以后逐渐下降 ,到固定 4周表达水平接近正常。结论　机械牵张力刺激可以导致内源性VEGF生成 。Objective To investigate the expression of VEGF mRNA during the midpalatal suture and the application of tensile forces in young rabbits. Methods Haas appliance was cemented onto the maxillary posterior dentition. The midpalatal suture was rapidly expanded for 14 days. The animals were sacrificed on 0d, 7d, 14d, 21d, 28d, 42d after the start of activation, respectively. Then, the levels of VEGF mRNA were detected by RT-PCR. Results The expression of VEGF mRNA in the experiment group increased significantly at 7-day's group and reached peak in 21-day's group,then gradually decreased. The level of VEGF mRNA was higher than that in the control group. Conclusion Mechanical strain regulates the expression of VEGF at transcription level.

关 键 词：张应力 骨缝牵张 血管内皮生长因子 逆转录聚合酶链反应 

分 类 号：R783.5[医药卫生—口腔医学]