卡拉胶寡糖对放射损伤小鼠T细胞功能和亚型的影响  

Effect of carrageenan-derived oligosaccharides on T cell function and subsets in irradiated mice

作　　者：李翊[1] 王海青[1] 

机构地区：[1]解放军第四○一医院放射科,青岛266071

出　　处：《中华放射医学与防护杂志》2005年第1期41-42,共2页Chinese Journal of Radiological Medicine and Protection

基　　金：山东省攻关计划基金资助项目 (0 3 10 70 12 0 )

摘　　要：目的　探讨卡拉胶寡糖对受X射线照射小鼠T细胞功能和亚型的影响。方法　采用直线加速器 6MVX射线一次全身照射BALB c小鼠 ,卡拉胶寡糖组照射前连续 14d和照射后继续给药 7d。检测小鼠脾脏淋巴细胞增殖反应 ,胸腺细胞表达CD6 9变化 ,外周血T细胞表达IL 2、IFN γ和TNF α变化及CD+4 CD+8比值。结果　卡拉胶寡糖能有效提高脾脏T淋巴细胞的增殖能力 ,胸腺T细胞表达CD6 9显著升高 ,使受照射小鼠外周血T细胞产生IL 2和IFN γ的能力显著增强 ,外周血T细胞表达TNF α的能力降低 ,并能显著升高外周血T细胞CD+4 CD+8比值。结论　卡拉胶寡糖对放射损伤小鼠的T细胞功能和亚型有明显调节作用。Objective To observe the effect of carrageenan-derived oligosaccharides on T cell function and subsets in irradiated mice. Methods The carrageenan-derived oligosaccharides were applied to BALB/c mice for 14 successive days before and 7 days after whole body irradiation with 6.0 Gy X-rays. The transformation response of splenic lymphocyte,the expression of CD 69 in thymocytes,IL-2,IFN-γ and TNF-α in T-cells of PBMC,and CD+ 4/CD+ 8 ratio of T lymphocytes were evaluated. Results The carrageenan-derived oligosaccharides increased ConA-stimulated transformation of splenic T lymphocytes of mice,characterized by marked up-regulation of CD 69 expression in thymic lymphocytes,significantly enhanced the ability of PBMC to produce IL-2 and IFN-γ,decreased the expression of TNF-α in T-cells of PBMC,and over-expressed CD+ 4 in T lymphocytes,followed by increment in CD+ 4/CD+ 8 ratio of T lymphocytes. Conclusion Carrageenan-derived oligosaccharides can markedly adjust T cell function and subsets in irradiated mice.\;

关 键 词：小鼠 T细胞功能 外周血T细胞 亚型 表达 CD4^+/CD8^+ 放射损伤 寡糖 卡拉胶 比值 

分 类 号：R818[医药卫生—放射医学]