三氧化二砷提高恶性淋巴瘤细胞放射敏感性的观察  

Radiosensitization of arsenic trioxide on malignant lymphoma cell lines

作　　者：聂林[1] 张洹[2] 李萍[1] 郭良君[1] 王奕鸣[1] 庄承海[1] 

机构地区：[1]暨南大学附属第一医院肿瘤科,广州510630 [2]暨南大学医学院血液病研究所

出　　处：《中华放射医学与防护杂志》2005年第1期43-45,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：广东省医学科学技术研究基金资助项目 (B2 0 0 2 0 69)

摘　　要：目的　研究三氧化二砷对恶性淋巴瘤细胞辐射敏感性的影响。方法　采用细胞染色方法观察细胞凋亡的形态 ,流式细胞仪检测细胞的DNA含量和Bcl 2蛋白的表达。结果　 0 5～ 2 0μmol L三氧化二砷和 1～ 4Gyγ射线均能抑制B淋巴瘤细胞株Raji细胞生长 ,诱导细胞凋亡 ,流式细胞仪检测Raji细胞亚G1 期细胞明显增多 ,Bcl 2蛋白的表达明显降低 ,呈现时间剂量依赖效应 ,联合应用比单独应用抑制作用显著。结论　三氧化二砷能够提高恶性淋巴瘤细胞的放射敏感性。Objective To investigate the radiosensitization of arsenic trioxide on malignant lymphoma cell lines. Methods Cell apoptosis situation, cellular DNA contents and Bcl-2 protein expression were determined by cell staining and flow cytometry. Results One to four Gy 60Co γ-irradiation and 0.5- 2.0 μmol/L As 2O 3 could inhibit Raji cell growth accompanied by the appearance of morphologic characteristics of apoptosis. The number of Sub-G 1 cells significantly increased and Bcl-2 protein expression in Raji cells was significantly downregulated as assayed by flow cytometry. The relationship between Bcl-2 protein the concentration of As 2O 3 and γ-radiation showed both dose- and time-dependent features; so did the number of Sub-G 1 cells. The inhibitory effect was much more evident when As 2O 3 and irradiation were applied combinatively than either one of them was used alone. Conclusion Arsenic trioxide is able to enhance radiation effect on malignant lymphoma cell lines. \;

关 键 词：三氧化二砷 瘤细胞 恶性淋巴瘤 放射敏感性 观察 RAJI细胞 流式细胞仪检测 生长 剂量 形态 

分 类 号：R733.1[医药卫生—肿瘤]