槲皮素联合顺铂对胃癌SGC-7901细胞增生和凋亡的影响  

Effects of quercetin combined with cisplatin on proliferation and apoptosis of human gastric cancer SGC-7901 cells

作　　者：王海忠[1] 王沁[2] 

机构地区：[1]兰州军区总医院肿瘤科,甘肃省兰州市730050 [2]兰州医学院附属一院消化内科,甘肃省兰州市730030

出　　处：《世界华人消化杂志》2005年第3期303-307,共5页World Chinese Journal of Digestology

摘　　要：目的:探讨槲皮素(quercetin,QU)联合顺铂(DDP)对胃癌SGC-7901细胞增生、凋亡及凋亡相关基因Bcl-XL mRNA表达的影响. 方法:用槲皮素和顺铂处理胃癌SGC-7901细胞后,应用光镜、电镜、MTT法、流式细胞仪和RT-PCR 技术研究胃癌细胞的形态学变化、生长抑制、诱导凋亡和对凋亡相关基因Bcl-XL mRNA表达的影响. 结果:药物作用于细胞24 h后,可看到较为典型的细胞凋亡形态学变化.DDP和QU均可以抑制胃癌SGC-7901 细胞增生或诱导其凋亡,且有时间和剂量依赖效应,联合应用具有协同效应.联合组的诱导凋亡率及增生抑制效应显著高于单用DDP组(P<0.01),干预48 h后,Bcl- XLmRNA的表达下调,QU可以加强下调作用. 结论:人胃癌SGC-7901细胞体外实验中,QU和顺铂可以抑制其增生并诱导凋亡,二者联合应用有一定的协同效应,且呈剂量依赖关系.AIM: To investigate the effects of quercetin combined with cisplatin on the proliferation and apoptosis of, and the expression of apoptosis-related gene Bcl-XL in human gastric cancer SGC-7901 cells. METHODS: After treated with quercetin (QU), cisplatin (DDP) or both (QU-DDP), the viability of SGC-7901 cells was evaluated by MTT assay. Cell cycle distribution and apoptosis were detected by flow cytometry. Cell morphology was examined under light microscope and electron microscope. The expression of Bcl-XL mRNA was detected by the reverse transcription polymerase chain reaction (RT-PCR). RESULTS: After exposure to different drugs for 24 h, SGC-7901 cells manifested typical morphological features of apoptosis. Both DDP and QU inhibited the proliferation and induced the apoptosis of SGC7901 cells in a time and concentration-dependent manner. QU combined with DDP synergistically enhanced cell death. The apoptosis was more pronounced in cells treated with both drugs (QU: 1μmol/L, DDP: 80 μmol/L) than DDP alone (80μmol/L) (19.93±0.07 vs5.16±0.11, P<0.01). After exposure to DDP for 48 h, Bcl-XL mRNA expression was down-regulated, which was promoted by QU. CONCLUSION: QU combined with DDP effectively inhibits proliferation and induces apoptosis of the human gastric cancer SGC-7901 cells in vitro in a dose-dependent manner.

关 键 词：增生 SGC-7901细胞 胃癌 顺铂 槲皮素 联合 诱导凋亡 RNA 形态学变化 生长抑制 

分 类 号：R735.2[医药卫生—肿瘤] R734[医药卫生—临床医学]