大鼠正常肝细胞与肝癌细胞磷脂酰胆碱及脂肪酸组成的比较研究  

Analysis of phospholipids and fatty acids composition of rat hepatoma cells

作　　者：李亚丽[1] 满洪升[2] 夏泉[1] 马克里[1] 崔肇春[1] 

机构地区：[1]大连医科大学生化教研室,辽宁大连116027 [2]大连医科大学分析测试中心

出　　处：《大连医科大学学报》2001年第4期249-251,共3页Journal of Dalian Medical University

摘　　要：目的 :探讨肝癌细胞的膜磷脂组分变化。方法 :采用高效薄板层析方法并配合无机磷的测定 ,比较了大鼠正常肝细胞与癌变细胞磷脂的组成及摩尔分数 ,用气相色谱法对其中含量最多的磷脂组分磷脂酰胆碱 (PC)的脂肪酸组成进行了测定。结果与结论 :肝癌细胞的PC含量明显减少 (P <0 .0 5 ) ,PC中长链及不饱和脂肪酸含量显著减少 (P <0 .0 5 )。Objective: To study the membrane phospholipid composition of rat hepatoma cells. Method: The mole fraction of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) in total phospholipids of CBRH-7919 hepatoma cells was compared to that of normal liver cells by high performance thin layer chromatography (HPTLC) and phosphorus assay. The composition and mole fraction of fatty acids of PC in hepatoma cells were also compared to those of normal ones with gas chromatographic analysis (GC analysis). Results and Conclusion: The PC content of membrane phospholipids in CBRH-7919 tumor cells, particularly in long chain and unsaturated fatty acids was significantly lower than that of normal cells.

关 键 词：薄板层析 气相色谱 磷脂 磷脂酰胆碱 脂肪酸 

分 类 号：R735.7[医药卫生—肿瘤]