中国耐多药结核分枝杆菌临床分离株rpoB基因突变特点  

Molecular Mutations of rpoB Gene of Multidrug Resistant Mycobacterium tuberculosis Isolates from China

作　　者：乐军[1,2] 曾而良[1] 谢建平[1] 李瑶[1] 梁莉[3] 王洪海[1] 

机构地区：[1]复旦大学生命科学学院遗传学研究所遗传工程国家重点实验室 [2]上海市肺科医院,上海200433 [3]上海市肺科医院

出　　处：《Acta Genetica Sinica》2004年第12期1332-1336,共5页

基　　金：国家"973"计划重大疾病防治基础研究 (编号 :G19990 5 410 4)~~

摘　　要：为阐明中国耐多药结核分枝杆菌临床分离株rpoB基因的突变特征 ,对 86株结核分枝杆菌临床分离株rpoB基因两个区域 ,包括 81个碱基利福平抗药性决定区 (rifampinresistancedeterminingregion ,RRDR)和V176F区进行序列测定。其中 72株耐多药分离株中的 6 5株rpoB基因的RRDR区存在 2 2种不同类型突变、2 1种点突变和一个插入突变。最常见的突变部位分别位于密码子 5 31(4 1% )、5 2 6 (4 0 % )和 5 16 (4 % ) ,10 %耐药分离株未检测到突变。鉴定了RRDR内 6个新的等位基因 ,以及RRDR外部区域 5个新的突变。To characterize rpoB mutations of multidrug resistant Mycobacterium tuberculosis clinical isolates from China,mutations in the 81 bp rifampin resistance determining region(RRDR) and mutation V176F locating in the beginning of the rpoB gene were analyzed by DNA sequencing.Eighty six Mycobacterium tuberculosis clinical isolates,including 72 rifampin resistant strains and 14 rifampin sensitive strains were sequenced.Six five mutations of 22 distinct kinds,21 point mutations and one insertion,were observed in 65 of 72 resistant isolates.The most frequent mutations were in codon 531(41%),526(40%),and 516(4%).Mutations were not found in seven(10%) of the resistant isolates.Six new alleles within the RRDR,along with five novel mutations outside the RRDR,are reported.None of isolates contained the V176 mutation.

关 键 词：结核分枝杆菌 耐药性 RPOB基因 突变频率 DNA序列分析 

分 类 号：R346[医药卫生—基础医学]