慢性复合应激对大鼠学习与记忆及海马NMDA受体NR1亚基表达的影响  

Effects of chronic complex stress on learning and memory and NMDA receptor subunit NR1 expression in hippocampus of rats

作　　者：程少容[1] 刘能保 张敏海[1] 刘少纯[1] 孙臣友[1] 李辉[1] 刘向前[1] 汪薇曦[1] 

机构地区：[1]华中科技大学同济医学院解剖学系组织胚胎学教研室

出　　处：《解剖学杂志》2004年第6期616-619,共4页Chinese Journal of Anatomy

基　　金：国家自然科学基金 (30 2 70 4 4 6 )

摘　　要：目的 :观察慢性复合应激对大鼠学习与记忆的影响和海马内NMDA受体亚基NR1表达的变化。方法 :成年雄性Wistar大鼠实验组每天交替暴露于复合应激原环境中达 6w ,然后作Morris水迷宫和Y迷宫作业测试 ,再采用免疫组织化学和图像处理方法分析海马CA1、CA3和齿状回区内NR1的表达变化。结果 :慢性复合应激组大鼠寻找平台的潜伏期较对照组明显缩短 ,学会躲避电击的正确次数较对照组明显增多 ;海马内NMDA受体亚基NR1的表达水平较对照组明显上调。结论 :慢性复合应激可增强学习与记忆能力 。Objective: To study the effects of chronic complex stress on learning and memory and NMDA receptor subunits NR1 expression in hippocampus of rats. Methods: Wistar male rats were randomly divided into control group and experimental group. The rats in experimental group were alternatively exposed to chronic complex stress for 6 weeks. After completion of the stress for experimental rats, both groups were tested with Morris water maze and followed with Y-maze. Afterwards, the brain tissue section were stained immunohistochemically to detect the expression changes of NR1 and the immunoreactivity within the CA1, CA3 and dentate gyrus of the hippocampus and was analyzed by computer-assisted image analysis system. Results: Compared with control group, the latent periods to search platform was significantly shortened in experimental rats and the times to correctly avoid the electric stroke was significantly increased in the experimental rats; the expression of NR1 was significantly up-regulated. Conclusion: Chronic complex stress might increase the ability of learning and memory. One of these mechanisms might be related with the expression changes of NMDA receptors.

关 键 词：慢性 NMDA受体 海马 NR1 学习与记忆 表达 大鼠 亚基 应激 体表 

分 类 号：R363[医药卫生—病理学]