重组色素上皮细胞衍生因子抑制鸡胚绒毛尿囊膜血管及兔角膜新生血管  

Recombinant pigment epithelium-derived factor for inhibition of neovascularization

作　　者：杨楠[1] 张桂荣[2] 杨翰仪[2] 府伟平[1] 

机构地区：[1]中国辽宁大连解放军210医院眼科,116021 [2]中国吉林大学白求恩医学部生化与分子生物学教研室,130021

出　　处：《国际眼科杂志》2004年第6期1009-1011,共3页International Eye Science

摘　　要：目的:观察制备的重组色素上皮细胞衍生因子(rPEDF)对鸡胚绒毛尿囊膜(CAM)血管及兔角膜新生血管的抑制作用。方法:制备rPEDF,采用鸡胚绒毛尿囊膜分析,观察rPEDF抑制新生血管生长情况。利用碱烧伤制作兔角膜新生血管模型,观察rPEDF抑制角膜新生血管生长状况。结果:rPEDF能明显抑制鸡胚绒毛尿囊膜血管生长。rPEDF治疗组兔角膜新生血管长度及生长面积明显少于对照组(P<0.05),差异有统计学意义。结论:制备的rPEDF抑制鸡胚绒毛尿囊膜血管生长,抑制兔角膜新生血管的形成。AIM: To evaluate the angiogenesis-inhibiting efficacy of recombinant pigment epithelium derived factor (rPEDF) for chick chorioallantoic membrane (CAM) and c orneal neovascularization (CNV). · METHODS: rPEDF was expressed primarily in E.coli BL21 by IPTG induction and p urified. By CAM and rabbit CNV model, the inhibiting effects of rPEDF under vari ous conditions were observed. · RESULTS: In CAM, the purified rPEDF showed a potent inhibitory activity on an giogenesis in vivo. In animal tests, the rPEDF also could inhibit strongly the g rowing speed and area of rabbit CNV compared to the controls (P <0.05). · CONCLUSION: It is demonstrated that rPEDF is a potent dose-dependent inhibito r of angiogenesis. ·

关 键 词：重组色素上皮细胞衍生因子 鸡胚 绒毛尿囊膜血管 角膜新生血管 抑制作用 

分 类 号：R770.5[医药卫生—眼科]