胃癌及癌旁组织EGFR和Ki-67抗原表达的免疫组化研究  

An Immunohistochemical Study of Expression of EGFR、Ki-67 in Human Gastric Carcinoma and Its Surrounding Tissues

作　　者：严辉[1] 陈卫昌[1] 蔡衍郎[1] 

机构地区：[1]苏州大学医学院附属第一医院 消化内科,江苏苏州215006

出　　处：《苏州大学学报（医学版）》2004年第6期855-857,860,共4页Suzhou University Journal of Medical Science

摘　　要：目的 研究胃癌及癌旁组织中EGFR和Ki-67抗原的表达情况及其与胃癌浸润、转移等生物学行为间的关系,从而探讨其对胃癌的早期诊断和临床治疗的价值。方法 运用免疫组织化学ABC法,检测48例胃癌及癌旁组织、86例胃良性疾病中EGFR和Ki-67的表达。结果 (1)胃癌和癌旁组织.EGFR、Ki-67呈高表达。(2)EGFR表达与胃癌生长方式、组织学分类、浸润深度、淋巴结转移与否及PTNM分期有关;Ki-67表达与胃癌的生长方式、浸润深度、淋巴结转移与否及PTNM分期无关,仅与组织学分类有关。结论 (1)EGFR和Ki-67的检测对胃癌的早期诊断有一定的参考价值。(2)癌旁组织显示增殖活性增高的趋势,显现出增殖不稳定状态,具有癌变可能。因此对胃癌患者行手术治疗时,应注意原发灶的切除范围,最大限度地减少残留病灶的可能性。Objective To explore the relationship between expression of EGFR、Ki-67 and the invasionm and metastasis of gastric carcinoma (GC) and its surrounding tissues; investigate its value in early diagnosis and assessing prognosis of patients with GC. Methods The expression of EGFR and Ki-67 was studied in 48 specimens resected from patients with GC and its surrounding tissues, 86 patients with benign gastric diseases by immunohistochemical ABC method. Results (1) Overexpression of EGFR and Ki-67 was detected in GC and its surrounding tissues. (2) The expression of EGFR in GC correlated with the growth pattern, histological type, depth of invasion, lymph node metastasis and the UICC TNM classification;the expression of Ki-67 in GC was independent of the growth pattern, depth of invasion, lymph node metastasis and the UICC TNM classification, but correlated with the histological type of the tumor. Conclusions (1)The expression of EGFR、Ki-67 may be helpful for early diagnosis with GC. (2) The adjacent tissues of gastric carcinoma possessed high proliferative activities for malignant transformation. Attention shonld be paid to the surgically resected extent and reduction of residual focus as far as possible.

关 键 词：胃癌 表皮生长因子受体 KI-67抗原 

分 类 号：R735.202[医药卫生—肿瘤]