小鼠髓系树突状细胞上4-1BB分子的功能性表达  

Expression of Functional 4-1BB Molecule on Mouse Myeloid Dendritic Cells

作　　者：李敏[1] 陈成[1] 张烽[1] 古涛[1] 朱一蓓[1] 於葛华[1] 马泓冰[1] 戴俊[1] 张学光[1] 顾宗江[1] 

机构地区：[1]苏州大学医学生物技术研究所 免疫学教研室,江苏苏州215007

出　　处：《苏州大学学报（医学版）》2004年第6期791-793,共3页Suzhou University Journal of Medical Science

基　　金：国家自然科学基金项目(30271205)江苏省临床免疫重点实验室基金资助课题

摘　　要：目的 探讨小鼠髓系DC(CD8α-)中4-1BB的功能性表达。方法 采用免疫荧光标记和流式细胞仪检测DC上4-1BB分子的表达,用酶联免疫方法检测DC分泌的IL-2和IL-12。结果 小鼠髓系DC前体细胞中已有4-1BB分子表达,且随着DC的分化发育和成熟,其表达上调。激发型抗4-1BB分子的抗体2A刺激的DC分泌IL-2和IL-12能力增强,而2A抗体联合TNF-α刺激的DC分泌IL-2和IL-12的量明显高于两者单独刺激组(P<0.05)。结论 小鼠髓系DC表达的4-1BB具有功能性。Objective To investigate 4-1BB expressions on mouse myeloid DC (CD8α-). Methods FCM was used to analyze the expression of 4-1BB on mouse myeloid DC and ELISA to determine the concentration of IL-2 and IL-12 in supernatant of DC. Results 4-1BB expressed on myeloid derived precursors and was up regulated during the process of DC maturation. Furthermore, DC stimulated by agonistic mAb to 4-1BB (2A) had efficient ability to secret IL-2 and IL-12. Moreover, DC stimulated by 2A together with TNF-α have more ffficient ability to secret IL-2 and IL-12. Conclusion Mouse myeloid DC expresses functional 4-IBB molecule.

关 键 词：树突状细胞 4-1BB 

分 类 号：Q254[生物学—细胞生物学] R392.1[医药卫生—免疫学]