应用基因芯片分析妇科恶性肿瘤的基因表达  

Application of the cDNA microarry in Analysis of gene expression patterns in malignant tumor of gynaecology

作　　者：易广才[1] 单祥年[2] 邵振堂[1] 

机构地区：[1]南京市妇幼保健院,南京210004 [2]南京师范大学生命科学学院,210097

出　　处：《中国优生与遗传杂志》2004年第6期3-7,共5页Chinese Journal of Birth Health & Heredity

基　　金：南京市跨世纪人才培养计划资助项目 (编号 :2 0 0 0 5 0 77-8)

摘　　要：目的　应用基因微矩阵芯片研究妇科恶性肿瘤相关基因表达。方法　分别提取癌组织和正常对照组织mR NA ,经反转录分别用Cy3-dCTP和Cy5 -dCTP荧光标记cDNA ,获得两组探针 ,混合后与基因芯片H4 0s(基因点数为 4 0 96点 )杂交 ,经严格洗片后用GenePix4 0 0 0B扫描仪进行扫描 ,获得荧光信号图像 ,用图像处理软件 :GenePixPro3.0对图像进行处理 ,获得两种组织中差异表达的基因信息。结果　在 4种癌组织中同时出现表达差异的基因有 35条 ,占 0 .85 %。其中 4种癌组织中都下调表达的基因 6条 ,没有均上调表达的基因 ,在前 3种癌组织中都上调表达而在乳腺癌中却下调表达的基因12条 ,在前 3种癌组织中都下调表达而在乳腺癌中却上调表达的基因 12条 ,其它 5条。结论　 1 肿瘤的发生过程比我们已了解的要复杂的多 ,它是多种基因表达失衡的结果。每种肿瘤的每个患病个体其肿瘤的发生和发展都具有自己一系列独特的基因表达谱 ,即使是同一肿瘤的不同个体之间 ,基因表达谱可能差异也很大。 2 子宫颈癌、乳腺癌、卵巢癌和子宫内膜癌这四种癌组织中具有相同表达行为的基因只有 6个 ,它们是 :AB0 2 3136 (编码人类蛋白KIAA0 919)、AF0 80 15 8(丝氨酸、苏氨酸K激酶基因 )、S795 2 2 (蛋白质合成延长因子EF - 1基因 )。Objective: To screen the differentially expressed genes in malignant tumor of gynaecology by cDNA microarry. Methods: The mRNA were isolated and purified from carcinoma and normal tissues then were reversely transcribed to cDNAs with the incorporation of fluorescent Cy3-dCTP or Cy5-dCTP one from the other to prepare two groups of hybridization probes , the mixed probes were hybridized to the cDNA microarray H40s(4096 gene points). After high-stringent washing, the cDNA microarry was scanned for the fluorescent signals by the scanner GenePix4000B, then the fluorescent signals were treated with image manipulation software GenePix Pro3.0 to show differentially expressed genes between each 2 tissues. Result: 35 of 4096(0.85%) genes were much different in expression level in four kinds of carcinoma tissues. 6 of 4096 were downregulated in four kinds of carcinoma, none of 4096 were upregulated genes,12 genes of 4096 were upregulated in cervical squamocellular carcinoma, ovarian malignant clear cell carcinoma and endometrial adenocarcinoma in uterus,while they were downregulated in breast duct soakage carcinoma.12 genes of 4096 were downregulated in cervical squamocellular carcinoma, ovarian malignant clear cell carcinoma and endometrial adenocarcinoma in uterus,while 5 of 4096 were upregulated in breast duct soakage carcinoma. Conclusion: 1.The origination and development of cancer is much more complicated than what we have known,it is the result to which the balance of many genes' expression were broken. The gene expression pattern is much different between various tumours or even same kind of tumour from different patients. 2.The similar expression pattern of 6 genes was observed in cervical squamocellular carcinoma, ovarian malignant clear cell carcinoma, endometrial adenocarcinoma in uterus and breast duct soakage carcinoma,they are : AB023136(Homo sapiens mRNA for KIAA0919 protein); AF080158 (Protein-Serine-Threonine Kinases); S79522 (The human gene sequences encoding the translation-associated functions

关 键 词：宫颈鳞癌 卵巢恶性透明细胞癌 乳腺导管浸润癌 子宫颈癌腺癌 基因表达谱 微矩阵基因芯片 

分 类 号：R737.3[医药卫生—肿瘤]