hTERT Promoter/Bax靶向性涎腺恶性肿瘤基因治疗系统的构建  

The Construction of hTERT Promoter/Bax Targeting Gene Therapy System for Malignant Salivary Gland Tumors

作　　者：苏涛[1] 孙宏晨[1] 欧阳喈[1] 

机构地区：[1]吉林大学口腔医学院病理科,吉林长春130041

出　　处：《口腔医学研究》2004年第6期567-569,共3页Journal of Oral Science Research

基　　金：20 0 3年度高等学校博士点基金资助 (编号 :2 0 0 3 0 183 0 68)

摘　　要：目的 :构建hTERTPromoter/Bax靶向性涎腺恶性肿瘤基因治疗系统。方法 :采用氯化钙法在大肠杆菌DH5α中扩增pACTERT -EGFP和 pACCMV -Bax质粒。DNA提取纯化试剂盒提取细菌中生长的质粒 ,经电泳 ,紫外分光光度计检验后用BamHI,EcoRI核酸内切酶分别对这两个质粒进行单酶酶切。琼脂糖凝胶电泳分离酶切片段 ,切取其中 8.38,0 .5 8kbp两个片段长度的凝胶。凝胶提取试剂盒提取其中所含DNA片断 ,T4DNA连接酶连接这两个片段 ,琼脂糖凝胶电泳检测连接结果。结果 :通过连接反应获得 8.96kbp的重组DNA片段。 结论 :成功构建hTERTPromoter/Bax重组表达单位。Objective: To construct the hTERT Promoter/Bax targeting gene therapy system for malignant salivary gland tumors. Methods: The pACTERT-EGFP and pACCMV-Bax plasmids were transferred into E. Coli. DH5αby CaCl 2 solution. The plasmids were extracted and purified. Then a single restriction enzyme(Bam HI, Eco RI)digestion was carried out separately to cut the two plasmids into two segments. These segments were separated by an agarose gel electrophoresis. Two segments (8.38kbp, 0.58kbp) were selected and extracted from the gel. T4DNA Ligase was added to these two segments'mixture. After a linkage process, the reconstruction fragment was testified by an agarose gel electrophoresis. Results: We got a 8.96kbp construction DNA fragment which is in accordance with theoretical calculation. Conclusion: The hTERT Promoter/Bax expression unit was successfully constructed.

关 键 词：pACTERT—EGFP pACCMV—Bax 涎腺肿瘤 基因治疗 

分 类 号：R739.87[医药卫生—肿瘤]