HIV复制型痘苗病毒载体疫苗免疫原性分析  

Immunogenicity analysis of HIV vaccine based on replicating vaccinia virus Tiantan vector

作　　者：刘颖[1] 段丹丽[1] 彭虹[1] 唐海丽[1] 刘沙[1] 张宁[1] 王宁[2] 刘建源[2] 邵一鸣 

机构地区：[1]中国疾病预防控制中心性病艾滋病预防控制中心病毒免疫室,北京100050 [2]北京生物制品研究所

出　　处：《中华实验和临床病毒学杂志》2004年第3期281-283,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的　分析比较HIV复制型痘苗病毒载体疫苗在小鼠和家兔体内的免疫原性。方法　HIV痘苗病毒载体疫苗vTKgpe以肌内、皮下、皮内 3种途径接种小鼠 ,每周采血检测HIV特异性抗体和针对痘苗病毒载体的抗体 ,4周时取脾细胞用流式细胞仪检测细胞免疫。此外vTKgpe皮内途径接种 2只家兔 ,每周采血检测抗体。结果　肌内和皮下免疫的小鼠在 2周时开始出现HIV特异性抗体 ,4周时开始消失 ;针对痘苗病毒载体的抗体滴度在 4周时急剧升高 ;血清吸附实验表明痘苗病毒抗体的升高对HIV特异性抗体检测有一定的掩盖。细胞免疫仅在皮内免疫的小鼠中检测到。家兔的HIV特异性抗体在 2周时出现 ,并能维持一段时间。结论　在小鼠体内 ,肌内和皮下注射 2种途径倾向于诱导体液免疫 ,而皮内接种则以诱导细胞免疫为主。家兔对痘苗病毒的敏感性要高于小鼠。Objective To investigate the immunogenicity of HIV vaccine vTKgpe based on Vaccinia Virus Tiantan vector in mice and rabbits Methods Mice were inoculated with HIV vaccine vTKgpe by intramuscular (i.m.), intradermal (i.d.) or subcutaneous (s.c.) injections Blood sample were collected every week, and then antibodies against HIV and vaccinia virus vector were detected At week 4, some mice were killed and cellular immune responses were examined by flow cytomer Additionally two rabbits were vaccinated subcutaneously, blood sample were tested as done with mice Results In mice im and sc groups, HIV specific antibodies emerged at week 2 and declined at week 4 Antibodies against vector elevated rapidly at week 4, and potentially affected HIV specific antibody detection Cellular immune responses were only detected in sc group Serum of rabbit showed that anti-HIV antibody appeared at week 2 and maintained for several weeks Conclusion Vaccine vTKgpe innoculated by im and sc routes inclined to induce humoral immune responses in mice, but in i.d. group, inclined to induce cellular immune responses Response to the recombinant vaccinia virus was more sensitive in rabbit than in mice

关 键 词：HIV 痘苗病毒载体 小鼠 特异性抗体 细胞免疫 皮下 体内 家兔 接种 疫苗免疫 

分 类 号：R392[医药卫生—免疫学]