人端粒酶逆转录酶与c-myc和刺激蛋白1在成釉细胞瘤中的表达  

Expression of Telomerase Activity and c-myc and Stimulatory Protein 1 in Human Ameloblastoma

作　　者：钟鸣[1] 李自娟[1] 王洁[1] 张波[2] 候琳[2] 宫雁冰[1] 

机构地区：[1]中国医科大学口腔医院病理科,辽宁沈阳110002 [2]北京大学医学部病理学系,北京100083

出　　处：《华西口腔医学杂志》2004年第6期499-502,共4页West China Journal of Stomatology

基　　金：辽宁省自然科学基金资助项目 (2 0 0 32 0 57)

摘　　要：目的　研究原癌基因转录因子c_myc和刺激蛋白 1(SP1)在人成釉细胞瘤 (AB)中的表达及与端粒酶逆转录酶 (hTERT)的关系。方法　选取 5 4例AB(原发 31例 ,复发 19例 ,恶变 4例 )存档蜡块 ,采用原位杂交检测AB中c_mycmRNA、hTERTmRNA的表达 ,采用免疫组化SP法 ,检测AB中SP1蛋白的表达。结果　c_mycmRNA在AB中阳性率为 81 5 % (44 5 4 ) ,hTERTmRNA为 94 4 % (5 1 5 4 ) ,SP1为 83 3% (45 5 4 ) ;伴随AB的复发与恶变 ,3者表达强度上升 ;相关性分析得出hTERT与c_myc之间存在相关性 (rs =0 85 3,P <0 0 0 1) ,hTERT与SP1之间存在相关性 (rs=0 90 0 ,P <0 0 0 1)。结论　hTERT在AB中明显增加的活性可能与转录因子c_myc和SP1有关 ,3者在AB中的表达趋势与AB的临床生物学特性相关。Objective To study the oncogene transcriptor c-myc, stimulatory protein 1 (SP1) expression in ameloblastoma (AB) and their relation with telomerase reverse transcripase (hTERT), and to investigate the clinical biological characteristics of AB.Methods The expression was observed in AB by in situ hybridization and SP method.Results The positive rates of c-myc mRNA, hTERT mRNA and SP1 protein were 81.5%(44/54)、94.4%(51/54) and 83.3%(45/54), respectively. Their positive rates increased as AB recurred and transformed malignantly. A strong correlation was found between hTERT and c-myc, hTERT and SP1 (r-s=0.853,P<0.001; r-s=0.900,P<0.001).Conclusion Activity of telomerase plays an important role in the tumorigenesis development of AB. Increasing of hTERT expression may be related to c-myc and SP1. The expression of these three parameters has a significant correlation with the clinical biological characteristics of AB.

关 键 词：成釉细胞瘤 牙源性角化囊肿 端粒酶逆转录酶 细胞癌基因 刺激蛋白1 

分 类 号：R739.8[医药卫生—肿瘤]