转染内皮型一氧化氮合酶基因的内皮细胞抗血小板聚集和抗平滑肌细胞增殖的早期研究  

Endothelial nitric oxide synthase gene transfected endothelial cells inhibit smooth muscle cell proliferation and platelet aggregation early in vitro

作　　者：薛冠华[1] 张纪蔚[1] 张岚[1] 张皓[1] 张柏根[1] 

机构地区：[1]上海第二医科大学附属仁济医院血管外科,200001

出　　处：《中华外科杂志》2004年第22期1349-1352,共4页Chinese Journal of Surgery

摘　　要：目的　检测转染内皮型一氧化氮合酶 (eNOS)基因的内皮细胞功能。方法　将不同实验犬内皮细胞分为 4组 ,对照组为正常内皮细胞、β gal组为转染 β gal的内皮细胞、eNOS组为转染eNOS的内皮细胞、L 硝基 精氨酸甲酯组为转染eNOS的内皮细胞加L 硝基 精氨酸甲酯。分别采用四甲基偶氮唑盐比色法 (MTT)和氚 胸苷掺入法 ( 3 H TdR) ,检测各组的内皮细胞抗血小板聚集和抗平滑肌细胞增殖效用。结果　血小板聚集程度在不同时间eNOS组与正常组相比明显降低 ,12 0h时正常组为 4 2 2 ,eNOS组为 32 6 (P <0 0 5 ) ;平滑肌细胞增殖在不同时间eNOS组与正常组相比明显降低 ,12 0h正常组和eNOS组MTT吸光值分别为 0 2 8和 0 2 2 (P <0 0 5 ) ,3 H TdR掺入值分别为 4 6 91ccpm和3995ccpm(P <0 0 5 )。结论　体外培养已转染eNOS基因的内皮细胞 ,可明显抑制实验犬血小板聚集和平滑肌细胞增殖 ;抑制效果在培养至 4 8h时已非常明显 ,至少可持续至 12 0h ;抑制效果可被L 硝基Objective To test the function of endothelial cells transfected eNOS gene. Methods To test the function of eNOS-overexpressing endothelial cells inhibiting smooth muscle cell proliferation and platelet aggregation in vitro by colorimetry, MTT and 3H-TdR permeate, respectively, in four different endothelial cells groups. Results The platelet aggregation is significantly decreasing between the groups of EC and eNOS at the different time ( P <0.05), is 42.2 and 32.6 seperately at the time of 120 h. The SMC proliferation is significantly decreasing between the groups of EC and eNOS at the different time ( P <0.05). It is 0.28 and 0.22 by MTT test, 4691 and 3995 by 3H-TdR permeate separately at the time of 120 h. Conclusions eNOS-overexpressing endothelial cells inhibited significantly smooth muscle cells proliferation and platelet aggregation in vitro; which shows powerful effection 48 hours post transfection and lasts up to 120 hours at the least; and were holden back by L-NAME.

关 键 词：内皮细胞 NOS 转染 平滑肌细胞增殖 正常 抗血小板聚集 实验犬 阻遏 四甲基偶氮唑盐比色法 基因 

分 类 号：R-5[医药卫生]