Ⅱ相代谢酶基因多态性与广西肝癌发生的关系  

Relationship between Polymorphisms of Phase Ⅱ Metabolic Genes and the Susceptibility to Hepatocellular Carcinoma in Guangxi

作　　者：贺菽嘉[1] 覃甲仁[1] 顾永耀[2] 钟卫干[3] 苏上贵[1] 

机构地区：[1]广西医科大学,530021 [2]广西医科大学病理学教研室,530021 [3]广西中医学院生物化学教研室,530001

出　　处：《实用癌症杂志》2004年第5期460-462,473,共4页The Practical Journal of Cancer

摘　　要：目的　探讨Ⅱ相代谢酶谷胱甘肽硫转移酶M 1、T 1(GSTM 1、GSTT1)及微粒体环氧化物水解酶 (mEH )基因多态性与广西肝癌易感性的关系 ,以及基因与基因间的相互作用。方法　采用多重PCR、PCR RFLP技术 ,对广西地区 10 5例肝癌患者及 15 1例健康对照的GSTM 1、GSTT 1、mEH基因型进行检测。结果　GSTM 1基因缺失率在病例组与对照组中分别为 64 .76%和5 0 .99% ,两者比较有显著性差异 (P <0 .0 5 ,OR =1.77) ;病例组GSTT 1基因缺失率 (4 0 .95 % )高于对照组 (3 3 .11% ) ,mEH 3种基因型频率在病例组分别为 2 7.62 %、2 1.90 %、5 0 .48% ,对照组则分别为 2 1.19%、3 4.44 %、44 .3 7% ,两组比较无显著性差异 (P >0 .0 5 ) ;GSTM 1、T 1基因同时缺失的个体患肝癌的危险性增大了 1.2 2倍。结论　GSTM 1、T1基因同时缺失是肝癌的易感因素 ,可作为肝癌高危人群筛选的标记物。Objective To study the relationship between the polymorphisms of phase Ⅱ metabolic genes(GSTM1、GSTT1、mEH) and the susceptibility to hepatocellular carcinoma(HCC) in Guangxi with a focus on the gene-gene interaction.Methods The genotypes of GSTM1、GSTT1 and mEH were analyzed in 105 patients with HCC and 151 healthy controls by multiplex PCR and PCR-RFLP.Results The rate of the GSTM1 null genotype was 64.76% for the HCC and 50.99% for the normal controls,showing a significant difference between them(P<0.05,OR=1.77).The frequency of the GSTT1 null genotype showed no difference between the HCC group and the control group(40.95% vs 33.11%,P>0.05).The frequencies of 3 genotypes for mEH were 27.62%、21.90% and 50.48% for the HCC group and 21.19%、34.44% and 44.37% for the control group,showing no marked difference between the two groups.The relative risk of HCC was found to be 1.22 for individual with both GSTM1 and GSTT1 deletion.Conclusion Population with both GSTM1 and GSTT1 gene deletion is susceptible to HCC.GSTM1 and GSTT1 can be used as markers for screening the population with a high risk of HCC.

关 键 词：肝癌 谷胱甘肽硫转移酶M1 谷胱甘肽硫转移酶T1 微粒体环氧化物水解酶 多态现象 

分 类 号：R735.7[医药卫生—肿瘤]