食管癌组织中p21^(cip1/WAF)、p53表达及HPV-16感染的意义  

Expression of p21 ^(cip1/WAF),p53 and the Infection of HPV-16 in the Esophageal Cancer

作　　者：张建中[1] 吕怀盛[1] 景丽[1] 秦憬[1] 

机构地区：[1]宁夏医学院病理学教研室,750004

出　　处：《实用癌症杂志》2004年第5期488-490,共3页The Practical Journal of Cancer

基　　金：宁夏回族自治区卫生厅科研基金资助 (2 0 0 0 )

摘　　要：目的　探讨食管癌发生原因及其机制。方法　采用免疫组化方法 ,检测食管癌组织中p2 1cip1/WAF、p5 3蛋白表达水平 ,同时检测HPV 16。并与正常食管上皮和癌旁非典型增生上皮进行对比观察。结果　 9例正常食管鳞状上皮中有 8例p2 1cip1/WAF阳性 (88.89% ) ,明显高于食管癌组织 (4 8.72 % ,19/3 9) ;非典型增生中其阳性率 (74.19% ,2 3 /3 1)介于两者之间 ,非典型增生者其阳性率与食管癌比较无显著性差异 (χ2 =3 .669,P >0 .0 5 ) ,但表达强度明显强于食管癌 (χ2 =7.40 3 ,P <0 .0 1) ;正常上皮中 p5 3表达均阴性 ,食管癌组织中p5 3阳性率 (64 .10 % )明显高于非典型增生上皮 (3 5 .48% ) ,P <0 .0 1;正常食管上皮中HPV 16均阴性 ,3 9例食管癌有 13例表达阳性 (3 3 .3 3 % ) ,与非典型增生 (9.68% )比较无显著性差异 (χ2 =3 .3 73 ,P >0 .0 5 )。结论　食管癌患者可发生HPV 16感染 ,食管癌发生与 p5 3基因突变以及 p2 1cip1/WAF表达减弱有关。Objective To investigate the etiology and mechanism of the development of esophageal cancer.Methods The expression of p21 cip1/WAF,p53 and HPV-16 infection were detected by immunohistochemistrical technique in the esophageal cancer and surrounding epithelium with atypical hyperplasia and in the normal epithelium.Results The positive staining of p21 cip1/WAF was significantly higher in normal esophagus squamous epithelium(88.89%,8/9)than in the carcinoma(48.72%,19/39).The positive rate of p21 cip1/WAF in the atypical hyperplasia(74.19%,23/31)was slightly higher,though insignificantly,than that in the carcinoma,but the intensity of positive staining was significantly stronger than that in carcinoma(χ2=7.403,P<0.01).The staining of p53 was negative in the normal epithelium.The positive staining of p53 in the carcinoma(64.10%) was significantly higher than that in the atypical hyperplasia(35.48%,P<0.01).There was no positive staining of HPV-16 in the normal group.The positive staining of HPV-16 was not significantly different between the esophageal cancer(33.33%,13/39) and atypical hyperplasia(9.68%).Conclusion HPV-16 could be infected in patients with esophageal carcinoma.The development of esophageal cancer is related to the mutation of p53 gene and the lower expression of p21 cip1/WAF.

关 键 词：食管癌 p21^cip1/WAF基因 p53基因 人类乳头瘤病毒 

分 类 号：R735.1[医药卫生—肿瘤]