异丙酚对大鼠缺氧性肺血管收缩反应的影响  

Effect of propofol on hypoxic pulmonary vasoconstriction in rats

作　　者：洪方晓[1] 李树人[1] 

机构地区：[1]首都医科大学附属北京友谊医院麻醉科,100050

出　　处：《中华麻醉学杂志》2004年第12期909-912,共4页Chinese Journal of Anesthesiology

摘　　要：目的 观察异丙酚对大鼠缺氧性肺血管收缩(HPV)反应的影响及可能机制。方法 雄性 SD 大鼠，麻醉后取出心肺并制成肺动脉环，给予不同的悬挂初张力，以得到鼠肺动脉环收缩的最适初张力，在此最适初张力下测定肺动脉环的 HPV 反应强度；此后，分别给予不同浓度的异丙酚(1、3、10、30、100μmol/L)测量 HPV 反应强度的变化。测量60mmol/L KCI 引起的肺动脉环收缩强度，并比较异丙酚(10、30μmol/L)预先给药后收缩强度的改变；累计剂量式加入去氧肾上腺素(PE)测量肺动脉环收缩强度，并与异丙酚(10、30μmol/L)预先给药后进行比较。结果 小剂量异丙酚对 HPV 反应无明显影响(P>0.05)，随着剂量的增加对 HPV 的影响加大(P<0.05)。异丙酚呈剂量依赖性抑制肺动脉对 KCl 的收缩反应(P<0.05)。异丙酚抑制肺动脉对 PE 的收缩反应与剂量有关，不仅对 PE 引起的最大收缩反应具有抑制作用，且导致肺动脉对 PE 的敏感性降低。结论 异丙酚剂量依赖性抑制鼠肺动脉环的 HPV 反应，其可能机制是异丙酚对于肺动脉平滑肌细胞内钙的敏感性、内钙释放以及多种钙离子通道的抑制作用。Objective To investigate the effect of propofol on hypoxic pulmonary vasoconstriction(HPV)and assess the underlying mechanism in rats.Methods Twenty male SD rats weighing 300-400 g were.anesthetizedwith intraperitoneal phenobarbital.Heart and lungs were removed after thoracotomy.Pulmonary.arterial rings 4 mmin length and 1.0-1.4 mm in diameter were prepared and suspended in Earl solution maintained at 37℃ with a pHof 7.40 and aerated with 20% O_2-5% CO_2-25% N_2.The rings were stimulated with phenylephrine(PE)10^(-6)mol·L^(-1) with different preloads(300,500,700,900,1 100 mg).The isometric tension of the arterial rings wasmeasured.The optimal preload was determined to be 900 mg which allowed best contractility.Hypoxia was inducedby aerating the solution with 95% N_2-5% CO_2 and the flow rate was adjusted to maintain PO_2 of the solution at40-20 mm Hg and pH at 7.40.HPV of the rings were recorded.Then propofol was added to achieve a finalconcentration of 1,3,10,30,100 μmol·L^(-1) and HPV was again induced and the changes in HPV wererecorded.In addition the effects of propofol(10,30 μmol·L^(-1))on vasoconstriction produced by KCI and PE werealso measured.Results The lower doses of propofol(<10 μmol·L^(-1))had no significant effect on HPV whileincreased doses(>10 μmol·L^(-1))significantly inhibited HPV(P<0.05).The vasoconstriction produced by PEand KCI was also significantly inhibited by propofol in a dose-dependent manner.The sensitivity of pulmonaryarterial ring to PE was also decreased by propofol.Conclusion Propofol inhibits HPV in a dose-dependentmanner.It's inhibitory effects on sensitivity and release of intracellular calcium and calcium channels may beinvolved in the underlying mechanism.

关 键 词：异丙酚 肺动脉 HPV 缺氧性肺血管收缩反应 大鼠 剂量依赖性 影响 可能机制 目的观 结论 

分 类 号：R96[医药卫生—药理学]