一株三唑磷降解菌mp-4的分离鉴定及降解特性的研究  

ISOLATION,IDENTIFICATION AND CHARACTERIZATION OF TRIAZOPHOS DEGRADING BACTERIUM mp-4

作　　者：戴青华[1] 张瑞福[1] 蒋建东[1] 顾立锋[1] 李顺鹏[1] 

机构地区：[1]南京农业大学生命科学学院农业部农业环境微生物工程重点开放实验室,南京210095

出　　处：《土壤学报》2005年第1期111-115,共5页Acta Pedologica Sinica

基　　金：国家863项目(2003AA241150);国家"十五"攻关项目(2002BA516A01);江苏科技攻关项目(BE2002345;BE2003343)资助

摘　　要：从长期经有机磷农药污染的土壤中分离到一株能高效降解三唑磷的菌株mp-4,通过生理生化实验和16 S rDNA同源性序列分析将该菌鉴定为苍白杆菌属(Ochrobactrum sp.).mp-4菌能以三唑磷为唯一碳源生长,对三唑磷的降解率为98.3%.在25～37℃、pH值为6.6时生长较好,27～32℃、pH7.5～8.8时有较好的降解性能.在水稻大田试验中,米壳的三唑磷残留去除率为91.9%,糙米的三唑磷残留去除率为100%.A triazophos degrading bacterium designated as mp-4 was isolated from soils that have long been subjected to organophosphate pollution. Strain mp-4 was identified as Ochrobactrum sp. based on its biochemical-physiological characters and the result of the 16 S rDNA homologue sequence analysis. Strain mp-4 can grow with triazophos as its sole carbon source and degrade it at a rate of 98.3%. The optimal growth temperature and pH for mp-4 are 30℃ and 6.6 respectively. At the temperature of 27～32℃ and pH of 7.5～8.8,mp-4 can degrade triazophos well. Field test results show that mp-4 can decrease the residue of triazophos in rice husk and brown rice by 91. 9% and 100%,respectively.

关 键 词：三唑磷 生长 分离鉴定 大田试验 残留 水稻 生理生化 去除率 降解特性 降解菌 

分 类 号：S482[农业科学—农药学] X172[农业科学—植物保护]