硝酸铅对大蒜根尖细胞有丝分裂的影响  

Effects of Pb(NO_3)_2 on Mitosis of Garlic Root Tip

作　　者：高扬[1] 石秀红[1] 何正飚[1] 郑易之[2] 

机构地区：[1]长春师范学院生物系,长春130032 [2]深圳大学生物系,深圳518060

出　　处：《吉林农业大学学报》2004年第6期603-605,共3页Journal of Jilin Agricultural University

基　　金：国家自然科学基金资助项目(59877004)

摘　　要：采用不同浓度硝酸铅分别处理大蒜根尖,试验结果表明:随着硝酸铅质量浓度的增加(0.01.0.1,1.5, 10,100μg/mL)和染毒时间(6,18,24 h)的延长,细胞的增殖率下降,有丝分裂指数逐渐降低,由12.5％降至 5.65％,而且在有丝分裂的细胞中还出现微核、滞后染色体、染色体桥等畸变现象。这说明铅对植物具有毒害 作用。The experiment of using different concentrations of Pb(NO3)2 to deal with garlic root tip was carried out. The results show that with the increase of concentration of Pb(NO3)2(0.01,0.1,l,5,10 and 100 μg/mL)and extension time of dyeing(6,18,24 h) , both cell increment rate and mitotic index (from 12.5% to 5.65%)decreased. Furthermore, some abnormal phenomena such as micronucleus, lagging chromosomes and aberration of chromosomal bridge were also observed during mitosis.

关 键 词：硝酸铅 大蒜 有丝分裂指数 微核 染色体畸变 

分 类 号：S633.401[农业科学—蔬菜学] X132[农业科学—园艺学]