肺癌患者FLK-1、LRP和MDR1的表达与临床研究  

Expression and clinical studies on FLK-1,LRP and MDR1 in lung cancer patients

作　　者：朱春荣[1] 夏学鸣[1] 王庆才[1] 康苏娅[1] 

机构地区：[1]苏州大学附属第一人民医院肿瘤科,江苏苏州215006

出　　处：《中国癌症杂志》2005年第1期29-32,共4页China Oncology

摘　　要：　　　目的:探讨血管内皮生长因子受体 (Flk 1),肺耐药蛋白 (LRP)基因以及多药耐药基因 (MDR1)蛋白与肺癌患者临床及病理指标的关系。方法:用免疫组化技术(ABC法)对原发性肺癌组织中三种基因的表达进行检测。结果: 70例肺癌中,非小细胞肺癌MDR1阳性率 49. 2% (29 /59),明显高于小细胞肺癌 (SCLC) 18. 2% (2 /11)(P<0. 05);非小细胞肺癌LRP阳性率 69. 5% (41 /59),明显高于小细胞肺癌 27. 3% (3 /11) (P<0. 05)。腺癌中MDR1与LRP的表达明显高于鳞癌(P<0. 05)。LRP与Flk 1在NSCLCs中共同表达 49. 2% (29 /59),LRP的表达与肺癌的组织学分级相关,MDR1和LRP的表达与肺癌的组织学类型有关,Flk 1与TNM分期相关,均有统计学意义(P<0. 05)。Flk 1+LRP均阳性、FLK 1+LRP+MDR1均阳性、中药治疗、复发与患者的生存率有关(P<0. 05)。结论:FLK 1、LRP和MDR1基因蛋白产物的检测对肺癌患者的诊治和预后评估有积极意义。Purpose:To investigate the effect of Flk-1、LR P and MDR1 genes in the carcinogenesis and development of lung cancer. Methods:The expression of Flk-1、LRP and MDR1 geneproteins in primary lung tumors were studied immunohistochemically. Results:of the 70 lung cancers, 29 cases (49.2%) were positive for MDR1 expression in NSCLCs and 2cases (18.2%) in small cell lung carcinoma(S CLC); 41cases (69.5%)had overexpression of LRP in NSCLCs and 3cases (27.3%) in S CLCs, there was a statistically significant correlation (P<0.05). The squamo us carcinoma was significantly different(P<0.05) from adenocarcinoma in the positive rate of MDR1 and LRP. The co-expression rate of LRP with Flk -1 in 59 cases NSCLCs was 49.2%. There was significant correlation between the expression of LRP and histological grade(P<0.05). There was statistically significant correlation between the expression of MDR1、LRP and histological ty pe, the expression of Flk-1 and TNM staging also(P<0.05). Flk-1、MDR1 and LRP genes showed co-expression in some of the lung tumors. In a multivariate analysis by Cox regression model, the overexpression of Flk-1+LRP 、Traditiona l Chinese Medical therapy and recurrence were independently associated with pati ent survival length(P<0.05). Conclusions:The detection of Flk-1、MDR1 and LRP geneproteins has positive effects on diagnosis prognosis and treatment of lung tumors.

关 键 词：FLK-1基因 LRP基因 MDR1基因 基因表达 免疫组化 肺癌 预后 

分 类 号：R734.2[医药卫生—肿瘤]