拓扑替康对鼻咽癌放射增敏机制的研究  

Study of mechanism of radiation sensitizing effect of topotecan on nasopharyngeal carcinoma

作　　者：文庆莲[1] 吴敬波[1] 范娟[1] 郎锦义[2] 王艳萍[3] 

机构地区：[1]泸州医学院附属医院肿瘤科,四川泸州646000 [2]四川省肿瘤医院放疗科,四川成都610041 [3]四川大学华西医院肺癌分子生物学实验室,四川成都610041

出　　处：《中国癌症杂志》2005年第1期49-51,共3页China Oncology

基　　金：四川省科技厅资助(编号: 99 41 290)。

摘　　要：　　　目的:研究拓扑替康(TPT)对人鼻咽癌裸鼠移植瘤放射增敏的机制。方法:把放射增敏实验各组[RT+TPT组(增敏组),TPT组(药物组),RT组(放射组),生理盐水组(对照组) ]肿瘤标本制备成单细胞悬液,用流式细胞仪分析细胞周期,凋亡指数以及凋亡相关基因p53、bcl 2、bax的表达。结果:增敏组增殖指数 ( 19. 3±1. 7 )%降低,凋亡指数增加,和药物组(25. 2±0. 3)%,放射组 (26. 1±0. 3)%,对照组 (35. 7±2. 5)%比较有统计学意义;p53,bcl 2,bax的表达,各处理组与对照组比较,差异均没有统计学意义。结论:改变细胞周期,促进凋亡可能是拓扑替康增敏作用在细胞水平的重要机制,但诱导凋亡可能不依赖p53,bcl 2,bax基因。Purpose:To study the radiosensitising mechanism of topotecan in cell and molecule level on Nasopharyngeal carcinoma.Methods:To detect cell cycle,apoptosis index and the expression s of apoptosis genes such as p53, bcl2, bax by FCM.Results:The proliferation index of RT20Gy +TPT 12.5mg/kg group was decreased, while the apoptosis index was increased, with statistical signifi cance by comparison with TPT and RT group. Meanwhile, the expressions of apoptos is genes, such as p53,bcl-2,bax had no statistic significance between each grou p and the control group. Conclusions:The mechanisms of radiosensitizion might be its act ion on cell cycle,proliferation index and stimulation of apoptosis.However,the i nduction apoptosis might be independent of P53,bcl-2 and bax genes.

关 键 词：拓扑替康 鼻咽癌 裸小鼠 放射增敏机制 

分 类 号：R73-361[医药卫生—肿瘤]