大鼠心肌发育相关基因在心肌梗死后的表达变化  

Cardiogenesis-related Gene Expression Changes after Myocardial Infarction in Rats

作　　者：姜涛[1] 王春梅[1] 来仪[1] 贾竹青[1] 刘永刚[2] 马康涛[1] 李刚[1] 周春燕[1] 

机构地区：[1]北京大学基础医学院生物化学与分子生物学系,北京100083 [2]北京大学第三医院心内科,北京100083

出　　处：《中国生物化学与分子生物学报》2004年第6期833-837,共5页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家高技术研究发展计划 (863计划 )基金 (2 0 0 1AA2 160 3 13 );北京市科技计划重大项目基金 (H0 2 0 2 2 0 0 10 490 );教育部 2 11工程和北京大学985基金资助项目~~

摘　　要：探讨大鼠急性心肌梗死后与心肌发育相关基因的表达变化 .通过结扎大鼠冠状动脉左前降支建立急性心肌梗死模型 ,利用逆转录聚合酶链式反应 (RT PCR)和Northern印迹技术分别检测GATA4、Nkx2 5BMP2和Wnt11基因在心肌梗死后不同时间的表达变化趋势 .结果显示 ,在正常心肌中 ,与心肌发育相关的基因GATA4、Nkx2 5和BMP2有较强的表达 ,而Wnt11基础表达较低 .心肌梗死后 ,GATA4、Nkx2 5和BMP2表达下降 ,7d后有所恢复 ;Wnt11的表达在心肌梗死后呈上升趋势 ,至梗死 7d达到高峰 .结果提示 ,心肌发育相关基因的表达在心肌梗死后有明显改变 ,并发现启动心肌发育的关键信号分子———Wnt11基因表达上调 。The expression profiles of GATA4, Nkx2.5, BMP2 and Wnt11 after myocardial infarction (MI) in rats were investigated by RT-PCR and Northern-blot analysis. GATA4, Nkx2.5 and BMP2 were highly expressed in normal rats. The basal expression of Wnt11 was low. After MI, the expressions of Nkx2.5, GATA4 and BMP2 were down-regulated markedly and recovered gradually after seven days. In contrast, the expression of Wnt11, which initiates heart development, was up-regulated, and reached the peak on day-seven after MI. The results indicated that heart developmental-related genes had different responses to the MI. The study provided an insight into cell differentiation under myocardial injury conditions.

关 键 词：心肌梗死 GATA4 NKX2.5 BMP2 Wnt11 

分 类 号：Q344[生物学—遗传学] R363[医药卫生—病理学]