心肌及传导组织内3种蛋白的变化与SMDS的相关性  

Study on the changes of actin, serum albnmin, fibronectin in cardiac cells and cardiac conducting system in cases with sudden manhood death syndrome

作　　者：罗斌[1] 罗质人[2] 汪冠三[2] 何韵[1] 唐双柏[1] 张斌[1] 林俊莲[2] 许心舒[2] 宋一璇[1] 毕启明[1] 

机构地区：[1]中山大学基础医学院法医系,广东广州510089 [2]广州市公安局刑科所,广东广州510030

出　　处：《中国法医学杂志》2004年第6期340-343,共4页Chinese Journal of Forensic Medicine

基　　金：国家自然科学基金(39700167)广东省医学科研基金(A1999124)卫生部辅助循环重点实验室项目(2002056)

摘　　要：目的 观察心肌及传导组织内肌动蛋白、血浆白蛋白及纤维连接蛋白的染色变化，探讨青壮年猝死综合征(SMDS)死后诊断的新方法。方法 应用免疫组化S-P法，对SMDS及冠心病猝死者心肌及传导系统内肌动蛋白、血浆白蛋白和纤维连接蛋白的进行染色观察。结果 22例SMDS例猝死者心肌及传导系统内的肌动蛋白缺染17例；血浆白蛋白染色阳性18例；纤维连接蛋白染色阳性15例。并发现在SMDS中，8例有CCS严重病变。结论 心肌及传导系统内血浆蛋白、肌动蛋白改变与SMDS密切相关，部分SMDS病例死前存在早期心肌缺血或梗死的改变。Objective To investigate the changes of actin (HHF35), serum albumin (A1), fibronectin (Fn) in cardiac cells and cardiac conducting sysytem (CCS) in order to probe into postmortem diagnosis of sudden manhood death syndrome (SMDS). Methods Immunohistochemical detections of actin, serum albumin and fibronectin (Fn) in the cardiac cells and CCS were performed by S-P method in cases with SMDS and cases died from coronary heart disease and non-cardiovascular diseases which were used as the control. Results Of the 22 cases with SMDS, 17 were found of actin-negative staining, 18 serum albumin-positive and 15 Fn-positive staining with evident pathological lesions in CCS in 8 cases. Conclusion It is suggested that SMDS is associated with early myocardial ischemia or infarction, which results in abnormality in actin, serum albumin and fibronectin (Fn) in the cardiac cells and CCS.

关 键 词：心肌 SMDS 组织内 血浆白蛋白 肌动蛋白 纤维连接蛋白 传导系统 目的观 结论 变化 

分 类 号：D919[医药卫生—法医学]