抗氧化中药对蛋白质非酶糖基化反应的影响  

Effect of Chinese Drugs with Anti-oxidation Function on Protein Non-enzyme Glycosylation

作　　者：孙敏[1] 孙晶[2] 朱荃[3] 

机构地区：[1]安徽大学生命科学院,安徽合肥230039 [2]江苏大学,江苏镇江212013 [3]南京中医药大学国家规范化中药药理实验室,江苏南京210029

出　　处：《南京中医药大学学报》2005年第2期103-105,共3页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：国家科技部"十五"攻关项目 (2 0 0 113A70 1A2 9)

摘　　要：目的　观察中药胡黄连和贯叶连翘对体外蛋白质非酶糖基化反应的影响 ,评价其抗氧化作用与非酶糖基化反应的关系。分析它们治疗糖尿病并发症的可能性。方法　将牛血清白蛋白和葡萄糖在体外共同孵育建立体外蛋白非酶糖基化反应体系 ,并将反应液分成对照组和不同的药物浓度组 ,在 37℃孵厢中孵育 6 0d ,以激发波长 (EX) 370nm/发射波长(EM) 4 4 0nm测定各组荧光值。结果　不同浓度的胡黄连和贯叶连翘以剂量依赖方式明显抑制AGEs的形成。结论　胡黄连和贯叶连翘可以抑制蛋白质的非酶糖基化 ,可能与其抗氧化作用有关。OBJECTIVE To observe the effect of Picrorhiza rhizome and Hypericum perforatum on in vitro protein non-enzyme glycosylation, evaluate the relationship between the anti-oxidation effect and non-enzyme glycosylation, and analyze the possibility of their use in the treatment of diabetes. METHOD BSA and glucose were used to produce an in vitro system for non-enzyme glycosylation, and the reaction fluid was divided into a control group and groups with different concentration, which were incubated for 60 days. The fluorescent value of each groups were then determined. RESULT The two drugs with different concentrations could markedly and dose-dependently inhibit the formation of AGEs. CONCLUSION The two drugs can inhibit the protein non-enzyme glycosylation, which is probably related to their anti-oxidation function.

关 键 词：非酶糖基化 氧化应激 晚期糖基化终末产物(AGEs) 抗氧化中药 胡黄连 贯叶连翘 

分 类 号：R285.5[医药卫生—中药学]