多药耐药基因在肝细胞癌中的表达及与p53、PCNA表达的相关性研究  

EXPRESSION OF MDR_( -1) GENE IN HEPATOCELLULAR CARCINOMA AND ITS CORRELATION WITH THE EXPRESSIONS OF p53 AND PCNA

作　　者：张秀梅[1] 李柏林[2] 宋敏[1] 宋继谒[1] 

机构地区：[1]中国医科大学病理教研室,沈阳110001 [2]中国医科大学化学教研室,沈阳110001

出　　处：《中国组织化学与细胞化学杂志》2003年第4期372-375,共4页Chinese Journal of Histochemistry and Cytochemistry

基　　金：辽宁省自然科学基金资助项目 (9810 5 0 0 10 2 )

摘　　要：目的　研究肝细胞癌 (HCC)组织中多药耐药基因MDR 1与p5 3蛋白及增殖细胞核抗原 (PCNA)表达的关系 ,旨在从基因水平进一步探讨预测化学治疗效果的可行性。方法　利用免疫组织化学方法 (S P法 )研究 30例肝穿活检的肝癌组织中MDR 1、p5 3和PCNA的表达。结果　 30例肝细胞癌中MDR 1的阳性表达率为 5 6 6 7% ,MDR 1阳性表达与组织学分级无关 (P >0 0 5 )。MDR 1表达与 p5 3、PCNA表达间无相关性 (P >0 0 5 )。结论　通过检测MDR 1基因可以对肝细胞癌病人进行化学治疗敏感性预测 ;肝细胞癌中MDR 1基因表达不依赖于 p5 3和细胞增殖这些因素。? Objective To correlate the expressionsof p53 and PCNA with the expression of MDR -1gene in hepatocellular carcinoma, and to evalulate the feasibility of predicting the response to chemotherapy with molecular indexs. Method The expressions of MDR-1, p53 and PCNA were examined by means of S-P immunohistochemical technique in 30 cases of hepatocellular carcinoma by puncturing tissues. Result The positive expression rate of MDR -1is 56.67% in the 30 cases of hepatocellular carcinoma, showing no correlation with the histologicalgrade(P>0.05)?There was no correlation between p53, PCNA and MDR -1gene expressions in hepatocellular carcionoma. Conclusion MDR -1provides a useful marker to predict chemotherapy sensitivity the expression of MDR -1gene seems to be independent of cell proliferation or the expression of p53.

关 键 词：肝细胞癌 多药耐药基因 P53 增殖细胞核抗原 免疫组织化学 

分 类 号：R735.7[医药卫生—肿瘤]