3-硝基丙酸预处理对大鼠局灶性缺血再灌注脑损伤的影响  

The effect of 3-nitropropionic acid preconditioning on cerebral ischemia-reperfusion in rats

作　　者：段东晓[1] 朱红灿[2] 孙圣刚[2] 李红戈[2] 肖伟忠[2] 童萼塘[2] 

机构地区：[1]郑州大学医学院生理学教研室,河南郑州450052 [2]华中科技大学同济医学院附属协和医院神经内科,湖北武汉430022

出　　处：《现代医学》2004年第3期147-150,共4页Modern Medical Journal

基　　金：教育部留学回国人员基金资助项目 (No .2 0 0 1 - 345)

摘　　要：目的　探讨线粒体毒素 3 硝基丙酸 (3 nitropropionicacid ,3 NPA)预处理对大鼠缺血 再灌注脑损伤的保护作用。方法　大鼠腹腔注射 3 NPA(2 0mg·kg-1) 3d后制作大脑中动脉缺血 再灌注模型 ,采用红四氮唑 (TTC)染色、荧光法和干湿重法观察 3 NPA预处理对缺血 2h再灌注 2 4h和 48h脑梗死体积、血脑屏障通透性和脑组织含水量的影响。结果　缺血 2h再灌注 2 4h和 48h ,3 NPA预处理组脑梗死体积变小 ,血脑屏障通透性降低 ,脑组织含水量减少 ,与对照组比较有显著性差异 (均P <0 .0 5 )。结论　 3 NPA预处理可以诱导脑缺血耐受 ,降低血脑屏障通透性、减轻脑水肿可能是其机制之一。Objective To investigate the effect of 3、nitropropionic acid preconditioning on cerebral ischemia-reperfusion in rats. Methods Rats were administrated either vehicle or 3、NPA at the dose of 20、mg·kg -1,intraperitoneal,3、days prior to a 2、h middle cerebral artery occlusion followed by 24、h or 48、h of reperfusion. Infarct volumes were assessed by a 2,3,5 triphenylte trazolinm chloride staining. The permerbility of blood brain barrier was measured by Evans blue extravasion. Brain water content was determined by dry wet weight method. Results Preconditioning with 3、NPA exhibited a reduction in infarct volume. While,the permerbility of blood brain barrier and brain water content in the 3、NPA preconditioning group had a distinct decrease as compared with the control group. Conclusions The preconditioning with 3、NPA induces cerebral ischemic tolerance,the mechanism of the development of 3、NPA、induced cerebral ischemic tolerance may relate to the decreased permeability of blood brain barrier and reduction of cerebral edema.

关 键 词：大鼠 血脑屏障通透性 缺血-再灌注脑损伤 3-硝基丙酸 局灶性 脑梗死体积 脑组织含水量 结论 显著性差异 目的 

分 类 号：R743[医药卫生—神经病学与精神病学] R285.5[医药卫生—临床医学]