环孢素A对大鼠脑缺血-再灌注损伤炎症反应的影响  

Effect of cyclosporin A on inflammation of cerebral ischemia-reperfusion-injury in rats

作　　者：肖伟忠[1] 孙圣刚[1] 朱红灿[1] 黎钢[1] 童萼塘[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院神经内科,湖北武汉430022

出　　处：《现代医学》2004年第3期159-162,共4页Modern Medical Journal

摘　　要：目的　探讨环孢素A对脑缺血 再灌注损伤的保护作用。方法　将 10 8只健康Wistar大鼠随机分为假手术组、生理盐水对照组和环孢素A组 ,参照Zea Longa线栓法制备局灶性脑缺血 再灌注模型 ,在脑缺血 2h再灌注 2 2h和 70h后 ,分别对各组各时间点大鼠的行为学评分 ,并对脑红四氮唑 (TTC)染色结果、缺血区白介素 1β(IL 1β)含量和髓过氧化物酶 (MPO)活性进行测定和分析。结果　在各时间点环孢素A组与生理盐水对照组相比 ,前者行为学评分优于后者(均P <0 .0 5 ) ;环孢素A组脑梗死灶体积比生理盐水对照组明显缩小 (均P <0 .0 5 ) ;环孢素A组局灶性缺血 再灌注后脑组织中IL 1β的含量明显降低 (均P <0 .0 1) ;环孢素A可显著抑制MPO的活性 (均P <0 .0 1)。假手术组与上述两组相比 ,各项观察指标无明显改变。结论　炎症反应参与脑缺血 再灌注损伤 ,环孢素A可显著降低缺血 再灌注后脑组织中IL 1β的含量、减轻缺血区内白细胞的浸润 ,对大鼠脑缺血 再灌注损伤具有明显的保护作用。Objective To explore the protective effect of cyclosporin A on the cerebral ischemia-reperfusion-injury in rats. Methods One hundred and eight Wistar rats were randomly divided into 3 groups. The shamed-operation group,normal saline control group and the cyclosporin A group. The focal cerebral ischemia-reperfusion-injury models were made by suture-occluded method. The score of behavior obstacle in rats were rated. TTC staining was used to calculate the infarct size. The activity of myeloperoxidease(MPO) and the content of interleukin、1β(IL、1β)were measured at 22、h and 70、h reperfusion following ischemia 2、h. Results Compared with normal saline control group,the cyclosporin A group got better score of behavior obstacle (all P< 0.05). The infarct size of cerebra was markedly decreased than that of the normal saline control group(all P< 0.05). The activity of MPO and the content of IL、1β of ischemia cerebral zone in cyclosporin A group were significantly lower than those in the normal saline control group(all P< 0.01).Compared with two reperfusion groups,the shamed-operation group had no obvious abnormal change in each measurement item.Conclusions Inflammatory response plays important role in cerebral ischemic reperfusion injury in rats. Cyclosporin A can reduce the content of IL、1β and inhibit the infiltration of leukocyte,which can provide protective effect on cerebral ischemic reperfusion injury in rats.

关 键 词：环孢素A 脑缺血-再灌注损伤 大鼠 对照组 IL-1Β 炎症反应 生理盐水 体积比 结论 时间点 

分 类 号：R743[医药卫生—神经病学与精神病学] R285.5[医药卫生—临床医学]