基于荧光猝灭原理的光纤化学传感器研究 Ⅱ.比较五种分子探针制成敏感膜的动力学猝灭性质  

Studies of Optic Chemical Sensors Based on Fluorescence Quenching Ⅱ. The Comparison of Dynamic Quenching for Five Kinds of Molecular Probe with Some Quenchers

作　　者：陈坚[1] 朱滨[1] 袁立懋 阿合买提江 李苹 

机构地区：[1]新疆医学院药学系药物分析教研室

出　　处：《新疆医学院学报》1993年第4期303-307,共5页

基　　金：国家自然科学基金(29160013);新疆维吾尔自治区科委(92920)联合资助

摘　　要：将芘、芘丁酸、对硝基苯芘丁酸酯、罗丹明123和乙二胺基萘磺酸钠等分子探针分别加入适当的固相支持剂和增塑稳定剂,制成五种敏感膜。测定了Cr^(3+)、I^-、甲硝唑和氯霉素等猝灭剂对各种敏感膜的动力学猝灭响应数据。实验结果显示分子探针的化学结构、电荷或取代基不同使敏感膜对猝灭剂有不同的选择性和灵敏度。Some molecular probes were added into the polymer and plasticizer which as a sensitive membrane used in fiber optic chemical sensor that can be measure some organic and inorganic compounds based on fluorescence quenching. In order to comparison of dynamic quenching for different molecular probe, pyrene, pyrenebutyric acid, nitrophenyl pyrenebutyrate, rhodamine 123 and AEANS were prepared on membranes and responded in some quenchers. The results show that the intensity of dynamic quenching was related with polarity, electric charge and chemical structure of fluorophors. They have different selectivity and sensibility for each quencher. Among them the membrane of PBA is excellent on reversibility, stability and sensitivity.

关 键 词：分子探针 敏感膜 荧光猝灭 传感器 

分 类 号：R912[医药卫生—药学]