急性脑缺血性小鼠免疫功能的变化  

The study on immune functions of mice with acute cerebral ischemia

作　　者：常全忠[1] 李东亮[1] 张淑玲[2] 

机构地区：[1]新乡医学院生理学教研室 [2]新乡医学院第一附属医院儿科

出　　处：《新乡医学院学报》1993年第4期259-261,共3页Journal of Xinxiang Medical University

摘　　要：研究目的探讨急性脑缺血性小鼠免疫功能的状况。处理方法以42只昆明种小鼠为研究对象,体重为23g～28g,雌雄不拘。随机分为一个对照组和两个实验组。在乙醚麻醉下阻断双侧颈总动脉,建立急性脑缺血动物模型。给对照组和其中一缺血组动物以生理盐水灌喂,每天10ml/kg,另一缺血组以地塞米松灌喂,每天17mg/kg。各组动物精心喂养。第6天,尾静脉给碳末悬液,测T-淋巴细胞数目和碳清廓指数及胸腺、脾脏的重量。结果小鼠急性脑缺血后,胸腺、脾脏的重量减轻,碳清廓能力下降,外周血中T-淋巴细胞数目减少。灌喂地塞米松后变化更明显。对照组与实验组有显著差异(P<0.05)。结论急性脑缺血后的小鼠免疫功能下降,且受免疫抑制剂地塞米松的影响。临床上,脑缺血后应注意糖皮质激素的合理应用,以免严重影响病人的自身免疫功能。Objective To exploremune functions of micejschemia.the conditions of im.with acute cerebra Methods Kunming mice,weight 23—28g,were used in this study.The experiment consistedof three groups(one control and two interven—tion groups).The experimental models of acutecerebral ischemia were made by ligating both sidesof cornmoll carotiel arteries (CCA)、underanesthetizatiOn with ether.The animals of controland one intervention group were fed with NS,1 0ml/kg everyday,by gastrogavage.The restanimals were ted with dexamethasone,I 7mg/kgeveryday,in the same method.All animals weregiven food and taken care of five days,and in—jected with solution of active C in the sixth day.The numbers of T——lymphocytes and the weighs of spleen and thymos and the clearnce of C were de-termined. Result The numbers of T——lymphocytes onthe peripherial blood and the weight of spleensand thymos and the clearance abilities of C de-creased.The differences be:tween control and in—tervention groups were significa'nt(P<0.05)Conelution The immune functions of mice with acute cerebral ischemia are declining and were affected by immune inhibi tol‘ as dexanlethasone.

关 键 词：小鼠 脑缺血 免疫功能 地塞米松 

分 类 号：R743.3[医药卫生—神经病学与精神病学]