嗜吡啶红球菌多氯联苯降解基因的克隆与表达  

Cloning and expression of polychlorobiphenyl/biphenyl degrading gene from Rhodococcus pyridinovorans

作　　者：孙艳[1] 杨秀清[1] 钱世钧[1] 

机构地区：[1]中国科学院微生物研究所,北京100080

出　　处：《中国环境科学》2004年第6期734-737,共4页China Environmental Science

基　　金：国家"863"项目(2002AA601170)

摘　　要：建立了多氯联苯/联苯降解菌嗜吡啶红球菌 R04 的基因组文库.活性筛选得到 1 个18kb的阳性片断,内含 1个 921个核苷酸的新的开环酶2,3-二羟基联苯双加氧酶基因 bphC2,该基因能够在大肠杆菌中表达,其蛋白分子量约为34kDa.此外,在该基因上游39bp处还发现另外一个 2,3-二羟基联苯双加氧酶基因 bphC1 的部分序列.A degrading genomic library of polychlorobiphenyl biphenyl in Rhodococcus pyridinovorans R04 was constructed. A 18 kb fragment was obtained with active screening, containing a new dihydroxybiphenyl dioxygenase gene (bphC2), which could be expressed in E. coli and its protein molecular weight was about 34 kDa. In addition, in 39 bp in upstream of the gene, there was another partial sequence of 2,3-dihydroxybiphenyl dioxygenase gene bphC1.

关 键 词：多氯联苯/联苯 生物降解 2 3-二羟基联苯双加氧酶 基因表达 

分 类 号：X172[环境科学与工程—环境科学]