半叶马尾藻多糖的提取和分析  

Extraction and Analysis of Sargassum Hemiphyllum Polysaccharides

作　　者：孟庆勇[1] 刘志辉[1] 徐美奕[1] 东野广智[1] 

机构地区：[1]广东医学院分析中心,广东湛江524023

出　　处：《光谱学与光谱分析》2004年第12期1560-1562,共3页Spectroscopy and Spectral Analysis

基　　金：广东省湛江市科技重点攻关项目 (9945)资助

摘　　要：采用热水浸提法提取半叶马尾藻多糖 [Sargassumhemiphyllum (Turner)C Ag polysaccharides ,SHP],并对其理化性质、提取率、含量、组成和性质进行了研究。SHP呈灰白色粉末状 ,溶于水 ,不溶于有机溶剂。碘 碘化钾反应呈阴性 ,说明提取物为非淀粉性多糖。提取率为 7 0 4 % ,总糖含量为 82 9%。紫外扫描结果表明多糖几乎不含核酸和蛋白质。红外光谱显示SHP主要为吡喃多糖 ,并显示多糖分子结构中存在 β 糖苷键。薄层层析结果提示该多糖可能为木聚糖。上述结果不仅说明该方法提取的物质是多糖 ,而且纯度好 ,提取效率高。Sargassum hemiphyllum polysaccharides(SHP) was extracted by thermal water method and the physical and chemical characters, the extraction rate, contents, compositions of SHP was studied. The result showed that SHP was ashen powder, water-soluble, insoluble in organic solvents. The reaction of iodide-potassium iodide was negative to evaluate that SHP was nonstarch polysaccharides. Extraction rate was 7.04% and the content of polysaccharides was 82.9%. Ultraviolet spectrum showed that there were little DNA and protein. Infrared spectroscopy showed that SHP was main pyrano polysaccharides and had P-linked glycopolysaccharides in molecule structure of SHP. Thin layer chromatography traced that SHP could be xylan. The results indicated that the extract was not only polysaccharides but also higher purify and the method was high efficiency.

关 键 词：半叶马尾藻多糖 阴性 SHP 薄层层析 提取物 含量 提取率 热水浸提法 木聚糖 淀粉 

分 类 号：O629.1[理学—有机化学] R818.74[理学—化学]