荔枝愈伤组织诱导及其体细胞胚胎发生研究初报  

A Prelimary Research Report of the Calliferous Litchi Induced and Somatic Embryogenesis in Vitro

作　　者：苏明申[1,2] 林顺权[3] 陈振光[4] 俞长河[4] 吕柳新[4] 

机构地区：[1]上海市农业科学院林木果树研究所 [2]福建农林大学园艺系福州350002 [3]华南农业大学园艺系 [4]福建农林大学园艺系

出　　处：《华南热带农业大学学报》2004年第4期1-4,共4页Journal of South China University of Tropical Agriculture

摘　　要：分别取元红荔枝盛花后45d未败育的幼胚、一个月龄的实生苗叶片、成龄树叶片、未受精子房接种在愈伤组织诱导培养基上,均能诱导出愈伤组织,但仅有幼胚能诱导出胚性愈伤组织。幼胚在含5% 蔗糖的MS基本培养基附加2,4-D2mg/L+水解乳蛋白500mg/L的培养基上诱导率最高。幼胚愈伤组织在含5% 蔗糖的基本培养基附加10% 椰乳的培养基上,白色胚状体发生率最高。胚状体易提前萌发,仅抽生根不抽生茎。健壮的未生根胚状体在含5% 蔗糖的培养基附加BA1mg/L的培养基上生根率最低,为理想的萌发培养基。随着BA浓度的降低,生根率提高,呈负相关。同浓度BA抑制生根的效果好于KT。在萌发培养基上生长一段时期后,少数较成熟的胚状体抽生出茎叶,长成完整植株。Immature embryos that were not sterile for 45 days after the period of full bloom, seedling leaves of 30 days old, adult tree leaves and unfertilized ovaries from latchi were respectively inoculated on MS medium added 2,4-D to induce calli. Calli could be induced from these explants, but embryogenic calli could only be induced from immature calli with the induction ratio reaching 41%, when immature calli were inoculated on MS medium added 5% sucrose, 2,4-D 2 mg/L and LH 500 mg/L. Embryogenic calli yielded most white embryoids when inoculated on MT medium added 5% sucrose and 10% CW(coconut milk water). Embryoids tended to germinate early, only rooting without stems and leaves. Vigorous embryois wiout roots were inoculated on MT medium added 5% sucrose and BA 1mg/L, and the rooting ratio was the lowest, thus an ideal germination medium .BA content negatively correlated with rooting ratio. The same BA content was better than KT to inhibit rooting. A little of relatively mature embryoids germinated stems and leaves after inoculated on germination medium for some period, and finally turned into whole plantlets.

关 键 词：幼胚 基本培养基 胚状体 生根 愈伤组织诱导 荔枝 初报 萌发 体细胞胚胎发生 负相关 

分 类 号：S512[农业科学—作物学] S513