麦草浆的木聚糖酶和漆酶/介体体系协同生物漂白研究  

Biobleaching of Wheat Straw Pulp by Synergistic Effect of Xylanase and Laccase/ Mediator System (LMS)

作　　者：冯文英[1] 常清荣[1] 李振岩[1] 

机构地区：[1]中国制浆造纸研究院,北京100020

出　　处：《中国造纸学报》2003年第2期60-63,共4页Transactions of China Pulp and Paper

摘　　要：用产自黑曲霉 (Aspergillusniger)的木聚糖酶和产自担子菌 (Basidiomycetesp )的漆酶进行了麦草浆次氯酸盐漂前预处理的研究。找出了木聚糖酶和漆酶不同处理程序对麦草浆硬度和漂白白度的影响 ,并对漆酶、介体用量和酶处理时间进行了优化。结果表明 ,漆酶用量为 5 0U/g ,介体ABTS用量为 0 1 % ,酶处理时间为 1 2 0min ;用LMS在 0 .1MPa氧压下处理麦草浆 ,纸浆硬度降低率比常压LMS处理浆提高2 1个百分点 ,即由 1 0 5%提高到 31 6 % ;经L X处理的纸浆硬度比X L低 ,漂白后纸浆白度分别比对照浆高 3 9%ISO和 3 0 %ISO。Sequential pretreatments of wheat straw Soda-AQ pulp with Aspergillus niger xylanase and Basidiomycete sp. laccase before sodium hypochloride bleaching were studied. The effect of different treatments by synergistic action of xylanase (X) and laccase (L) on pulp hardness (KMn04 number) and brightness was discussed, and the optimum conditions concerning laccase dosage, mediator dosage and reaction time were also investigated. Results showed that the optimum conditions were laccase charge 2.5 U/g, mediator charge 0.1% and retention time 120 min.The KMn04 number decreased rate of the pulp treated by LMS under 1 kg/cm2 oxygen pressure was 31.6%, 21 percent higher than that treated without oxygen. The hardness of the pulp pretreated by L-X sequence was lower than that by X-L one, their bleached pulps brightness was respectively 3.9%ISO and 3.0%ISO higher than the control pulp.

关 键 词：麦草浆 漆酶/介体体系 生物漂白 酶处理 纸浆 木聚糖酶 漂前预处理 硬度 ABTS 研究 

分 类 号：TS745[轻工技术与工程—制浆造纸工程] TS727