外周神经参与室旁核对大鼠胃缺血-再灌注损伤的调控作用  

Peripheral nerves participate in the regulation of paraventricular nucleus on gastric ischemia-reperfusion injury in rats

作　　者：张咏梅 阎长栋 周秀萍 祁友键 

机构地区：徐州医学院生理学教研室，江苏徐州221002

出　　处：《徐州医学院学报》2003年第3期193-196,共4页Acta Academiae Medicinae Xuzhou

基　　金：国家自然科学基金资助课题(基金编号 39970 2 81);; 江苏省教育厅自然科学基金资助课题(基金编号99KJB310 0 0 5 )

摘　　要：目的探讨迷走神经、交感神经在电刺激下丘脑室旁核(paraventrieular nucleus，PVN)对大鼠胃缺血一再灌注损伤(gastricr ischemia-repeifiasion injury，GI-RI)调控中的作用。方法采用夹闭大鼠腹腔动脉30min，松开动脉夹血流复灌lh的GI-RI模型和核团内电刺激的方法，观察切断膈下迷走神经或切除腹腔交感神经节及应用受体阻断剂后对电刺激下丘脑室旁核调控大鼠胃GI-RI的影响。结果切断双侧膈下迷走神经，切除腹腔交感神经节后再电刺激PVN+GI-RI，损伤较电刺激组分别减轻69．02％、55．16％，差异具有显著性(P<0．01)；静脉给予β-受体阻断剂心得安能完全消除电刺激PVN引起的GI-RI的保护作用(P<0．01)，损伤较电刺激组加重211．7％；给予M-受体阻断剂阿托品、α-受体阻断剂酚妥拉明不影响电刺激PVN引起的GI-RI的保护作用。结论迷走神经、交感神经参与了PVN对GI-RI的保护作用。Objective To investigate the role of the peripheral nerves in the regulation of paraventricular nucleus (PVN) on gastric ischemia-reperfusion injury (GI-RI). Methods Male SD rats were used to form GI-RI models by clamping the celiac artery for 30 minutes followed by reperfusion for 1 hour after removing the clamp. The regulatory mechanism of the PVN elicited by nuclear electric stimulation was examined under various conditions of the vagua and sympathetic nerve. Results When the PVN was stimulated after vagotomy and sympathectomy, the extent of GI-RI was reduced by 69.02% and 55.16% respectively (P<0.01). When β-R antagonist propranolol was given iv, the protrctive effect of of PVN stimulation on GI-RI would be abolished and the lesion became increased by 211.7%. Intravenous injection of M-R antagonist atropine orα-R antagonist phentolamine did not alter the protective effect of PVN stimulation on GI-RI. Conclusion The protective effect of PVN on GI-RI might be mediated through the vagus and sympathetic nerve.

关 键 词：室旁核 胃缺血 再灌注损伤 迷走神经 交感神经 大鼠 

分 类 号：R364.12[医药卫生—病理学]